Monomeric type I and type III transforming growth factor-β receptors and their dimerization revealed by single-molecule imaging

Cell Research
Wei ZhangYe-Guang Chen

Abstract

Transforming growth factor-β (TGF-β) binds with two transmembrane serine/threonine kinase receptors, type II (TβRII) and type I receptors (TβRI), and one accessory receptor, type III receptor (TβRIII), to transduce signals across cell membranes. Previous biochemical studies suggested that TβRI and TβRIII are preexisted homo-dimers. Using single-molecule microscopy to image green fluorescent protein-labeled membrane proteins, for the first time we have demonstrated that TβRI and TβRIII could exist as monomers at a low expression level. Upon TGF-β1 stimulation, TβRI follows the general ligand-induced receptor dimerization model for activation, but this process is TβRII-dependent. The monomeric status of the non-kinase receptor TβRIII is unchanged in the presence of TGF-β1. With the increase of receptor expression, both TβRI and TβRIII can be assembled into dimers on cell surfaces.

References

Jan 1, 1990·Journal of Cell Science. Supplement·F T BoydJ Massagué
Jul 1, 1994·The Journal of Cell Biology·Y I HenisH F Lodish
Jul 2, 1993·Cell·F López-CasillasJ Massagué
Apr 25, 1997·The Journal of Biological Chemistry·R G WellsH F Lodish
Oct 6, 1998·Annual Review of Biochemistry·J Massagué
Oct 6, 2000·Genes & Development·J W Harbour, D C Dean
Nov 1, 2000·Cell·J Schlessinger
Jun 18, 2003·Cell·Yigong Shi, Joan Massagué
May 8, 2004·Trends in Biochemical Sciences·Peter ten Dijke, Caroline S Hill
Mar 21, 2007·Nature Methods·Maximilian H Ulbrich, Ehud Y Isacoff
Dec 18, 2007·Nature Structural & Molecular Biology·Susy C KohoutEhud Y Isacoff
Mar 8, 2008·The Journal of Physical Chemistry. B·Zeyu XiaoXiaohong Fang
Sep 2, 2008·Proceedings of the National Academy of Sciences of the United States of America·Wei JiLiangyi Chen
Aug 4, 2009·Trends in Cell Biology·Jong Seok KangRik Derynck
Sep 2, 2009·Proceedings of the National Academy of Sciences of the United States of America·Wei ZhangYe-Guang Chen

❮ Previous
Next ❯

Citations

Sep 25, 2012·Analytical and Bioanalytical Chemistry·Wangxi LuoXiaohong Fang
Jun 28, 2011·Cell and Tissue Research·Yabing MuMaréne Landström
Mar 17, 2012·Cell & Bioscience·Fei Huang, Ye-Guang Chen
Jan 22, 2013·Annual Review of Physical Chemistry·Tie XiaXiaohong Fang
Feb 2, 2012·FEBS Letters·Marcelo EhrlichYoav I Henis
May 13, 2015·Progress in Biophysics and Molecular Biology·Yahong SunXiaohong Fang
Nov 20, 2013·The Journal of Physical Chemistry. B·Tie XiaXiaohong Fang
Jul 28, 2016·Experimental Eye Research·Cynthia L Pervan
Dec 1, 2017·Acta Biochimica Et Biophysica Sinica·Rong ZhaoXiaohong Fang
Dec 1, 2017·Acta Biochimica Et Biophysica Sinica·Feng XieLong Zhang
Mar 23, 2011·The EMBO Journal·Tao HuangAndrew P Hinck
Feb 1, 2014·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Ryan J Arant, Maximilian H Ulbrich
Oct 30, 2016·Molecular Nutrition & Food Research·Martina MaywaldLothar Rink
Dec 12, 2017·Acta Biochimica Et Biophysica Sinica·Xiaohua YanYe-Guang Chen
Apr 2, 2020·Annual Review of Analytical Chemistry·Fang LuoXiaohong Fang
Dec 19, 2014·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Franziska FrickeMike Heilemann
Jul 28, 2016·Cold Spring Harbor Perspectives in Biology·Akiko Hata, Ye-Guang Chen
Oct 4, 2019·Analytical Chemistry·Rong ZhaoXiaohong Fang
Apr 6, 2019·Journal of the American Chemical Society·Jiachao XuXiaohong Fang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.