Mononucleated Blood Cell Populations Display Different Abilities To Transmit Prion Disease by the Transfusion Route

Journal of Virology
Jean-Yves DouetOlivier Andréoletti

Abstract

Previous experiments carried out in a sheep scrapie model demonstrated that the transfusion of 200 μl of prion-infected whole blood has an apparent 100% efficacy for disease transmission. These experiments also indicated that, despite the apparent low infectious titer, the intravenous administration of white blood cells (WBC) resulted in efficient disease transmission. In the study presented here, using the same transmissible spongiform encephalopathy (TSE) animal model, our aim was to determine the minimal number of white blood cells and the specific abilities of mononucleated cell populations to transmit scrapie by the transfusion route. Our results confirmed that the transfusion of 100 μl, but not 10 μl, of fresh whole blood collected in asymptomatic scrapie-infected donor sheep can transmit the disease. The data also show that the intravenous administration of 10(5) WBCs is sufficient to cause scrapie in recipient sheep. Cell-sorted CD45R(+) (predominantly B lymphocytes), CD4(+)/CD8(+) (T lymphocytes), and CD14(+) (monocytes/macrophages) blood cell subpopulations all were shown to contain prion infectivity by bioassays in ovine PrP transgenic mice. However, while the intravenous administration of 10(6) CD45(+) or CD4(+)/8(+...Continue Reading

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Citations

Dec 1, 2019·Scandinavian Journal of Immunology·Baizhuo ZhangLi Cui
Jan 20, 2018·Journal of Risk Research·Kumanan WilsonJennifer Keelan
Jan 24, 2018·Vox Sanguinis·C R SeedL Cervenakova
Feb 4, 2021·Acta Neuropathologica·Jean-Yves DouetOlivier Andreoletti

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