Abstract
Previous experiments carried out in a sheep scrapie model demonstrated that the transfusion of 200 μl of prion-infected whole blood has an apparent 100% efficacy for disease transmission. These experiments also indicated that, despite the apparent low infectious titer, the intravenous administration of white blood cells (WBC) resulted in efficient disease transmission. In the study presented here, using the same transmissible spongiform encephalopathy (TSE) animal model, our aim was to determine the minimal number of white blood cells and the specific abilities of mononucleated cell populations to transmit scrapie by the transfusion route. Our results confirmed that the transfusion of 100 μl, but not 10 μl, of fresh whole blood collected in asymptomatic scrapie-infected donor sheep can transmit the disease. The data also show that the intravenous administration of 10(5) WBCs is sufficient to cause scrapie in recipient sheep. Cell-sorted CD45R(+) (predominantly B lymphocytes), CD4(+)/CD8(+) (T lymphocytes), and CD14(+) (monocytes/macrophages) blood cell subpopulations all were shown to contain prion infectivity by bioassays in ovine PrP transgenic mice. However, while the intravenous administration of 10(6) CD45(+) or CD4(+)/8(+...Continue Reading
References
Oct 24, 1996·Nature·J CollingeA F Hill
Oct 23, 1997·Nature·M E BruceC J Bostock
Dec 22, 1999·Transfusion·P BrownW N Drohan
Oct 21, 2000·Lancet·F HoustonC J Bostock
Nov 22, 2000·The Journal of General Virology·O AndréolettiF Lantier
Feb 14, 2004·Lancet·C A LlewelynR G Will
Aug 11, 2004·Lancet·Alexander H PedenJames W Ironside
Oct 14, 2004·Haemophilia : the Official Journal of the World Federation of Hemophilia·J W Ironside, M W Head
Dec 25, 2004·The Journal of Biological Chemistry·Cécile FéraudetJacques Grassi
Oct 22, 2005·Proceedings of the National Academy of Sciences of the United States of America·Annick Le DurHubert Laude
Mar 21, 2007·Emerging Infectious Diseases·Jean-Noël ArsacThierry Baron
Apr 12, 2007·Archives of Virology·C R MorenoD Calavas
Oct 28, 2008·Veterinary Research·Mark Edward ArnoldGerald Arthur Henry Wells
Jan 28, 2009·Transfusion·Jean-Jacques Lefrère, Patricia Hewitt
Feb 12, 2009·BMC Veterinary Research·Hugh A SimmonsAnthony E Wrathall
Jan 15, 2010·Haemophilia : the Official Journal of the World Federation of Hemophilia·A PedenJ W Ironside
Mar 12, 2010·Journal of Virology·Candace K MathiasonEdward A Hoover
Feb 25, 2011·PLoS Pathogens·Olivier AndréolettiCaroline Lacroux
Aug 23, 2011·PloS One·Sandra McCutcheonJean C Manson
Dec 14, 2011·Journal of Virology·Caroline LacrouxOlivier Andréoletti
Jun 28, 2012·PLoS Pathogens·Olivier AndréolettiCaroline Lacroux
Aug 4, 2012·PloS One·Caroline LacrouxOlivier Andréoletti
Nov 6, 2013·Nature Reviews. Immunology·Adriano AguzziCaihong Zhu
Jan 1, 2014·Emerging Infectious Diseases·Jean Yves DouetOlivier Andreoletti
Feb 28, 2014·Journal of Virology·Jean-Yves DouetOlivier Andreoletti
Nov 18, 2014·Current Opinion in Hematology·Jean Yves DouetOlivier Andréoletti
Citations
Dec 1, 2019·Scandinavian Journal of Immunology·Baizhuo ZhangLi Cui
Dec 18, 2019·The Journal of General Virology·Erin E McNultyCandace K Mathiason
Jan 20, 2018·Journal of Risk Research·Kumanan WilsonJennifer Keelan
Jan 24, 2018·Vox Sanguinis·C R SeedL Cervenakova
Dec 1, 2017·Pathogens·Neil A Mabbott
Feb 4, 2021·Acta Neuropathologica·Jean-Yves DouetOlivier Andreoletti
Nov 17, 2020·Access Microbiology·Najiba MammadovaJustin J Greenlee
Feb 19, 2021·PLoS Pathogens·M Khalid F SalamatE Fiona Houston