Monooxygenation of rifampicin catalyzed by the rox gene product of Nocardia farcinica: structure elucidation, gene identification and role in drug resistance

The Journal of Antibiotics
Yasutaka HoshinoJun Ishikawa

Abstract

We demonstrated that the rox gene of Nocardia farcinica encodes a rifampicin monooxygenase capable of converting rifampicin to a new compound 2'-N-hydroxy-4-oxo-rifampicin with a markedly lowered antibiotic activity. The deletion mutation (Deltarox) of the rox gene gave no significant influence to the rifampicin resistance of N. farcinica. However, transformation with a plasmid containing an overexpressing the rox gene markedly raised the rifampicin resistance in the strain with the deletion mutation of the rpoB2 gene as the principal rifampicin resistance determinant. On the other hand, rifampicin was decolorized by the wild-type strain, whereas it remained intact when incubated with the Deltarox strain. Based on these results, it will be conclusive that the rox gene is capable of initiating rifampicin degradation with a new metabolite formation at the first step and having a role as the secondary rifampicin resistance factor in N. farcinica.

References

Oct 15, 1975·Journal of the American Chemical Society·M L Casey, H W Whitlock
Jun 1, 1994·The Journal of Antimicrobial Chemotherapy·K YazawaS Iwasaki
Jun 1, 1993·Antimicrobial Agents and Chemotherapy·K YazawaS Iwasaki
Jan 1, 1996·Microbiology and Immunology·Y TanakaS Iwasaki
Oct 7, 2004·Proceedings of the National Academy of Sciences of the United States of America·Jun IshikawaMasahira Hattori
Mar 30, 2006·Antimicrobial Agents and Chemotherapy·Jun IshikawaHiroyuki Satoh
May 10, 2007·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar

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Citations

Jul 18, 2012·Antimicrobial Agents and Chemotherapy·Peter SpanogiannopoulosGerard D Wright
Apr 30, 2014·Proceedings of the National Academy of Sciences of the United States of America·Peter SpanogiannopoulosGerard D Wright
Aug 15, 2014·The Journal of Antibiotics·Beth P Goldstein
Dec 31, 2011·Biochimica Et Biophysica Acta·Stefania Montersino, Willem J H van Berkel
Dec 14, 2011·Applied Biochemistry and Biotechnology·Sushila MaharjanJae Kyung Sohng
Sep 30, 2010·Plasmid·Mario C Salinas-Carmona, María R Rocha-Pizaña
Aug 29, 2013·Environmental Microbiology·Quan LuoAlexander Steinbüchel
Mar 24, 2016·Proceedings of the National Academy of Sciences of the United States of America·Xiaofeng QiPeng Zhang
Mar 13, 2014·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·P SinghS T Cole
Oct 6, 2016·PloS One·Heba AbdelwahabPablo Sobrado
Dec 21, 2017·The ISME Journal·Andrew C PawlowskiGerard D Wright
Apr 12, 2017·Antimicrobial Agents and Chemotherapy·Dinah Binte AzizThomas Dick
Aug 26, 2016·The Journal of Biological Chemistry·Li-Kai LiuJohn J Tanner
Feb 9, 2016·Current Protocols in Microbiology·Dipesh DhakalJae Kyung Sohng
Jan 19, 2021·Archives of Biochemistry and Biophysics·Renata A G ReisPablo Sobrado
Apr 21, 2021·Accounts of Chemical Research·Matthew D SuretteGerard D Wright
May 1, 2021·International Journal of Molecular Sciences·Rustam Aminov
Apr 29, 2020·EcoSal Plus·Hamed Mosaei, Nikolay Zenkin
Oct 21, 2017·Microbiology Spectrum·Steeve GiguèreJennifer M Willingham-Lane
Aug 18, 2021·The Journal of Antibiotics·Rebekah A AdamsMark P Brynildsen

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