PMID: 7540640Jun 9, 1995Paper

Monopalmitic acid-peptide conjugates induce cytotoxic T cell responses against malarial epitopes: importance of spacer amino acids

Journal of Immunological Methods
A F VerheulA A Lal

Abstract

Cytolytic T cells (CTL) play a critical role in providing protection against the liver stage of malaria infection. Previous investigations have shown that induction of CTL against peptide or proteins can be achieved by attachment of lipids. In the present study, we used the Plasmodium berghei circumsporozoite protein CTL epitope (SYIPSAEKI (PL76)). This peptide with cysteine-serine (CS) as spacer amino acids was coupled to palmitic acid (PA). The same CTL epitope containing only an extra serine was linked to S-[2,3-bis(palmitoyloxy)-(2-RS)-propyl]-N-palmitoyl-(R)-cysteine (tripam-C). Inbred mice [(BALB/c x C57BL/6)F1] were immunized intravenously with the lipopeptides. Both types of lipopeptides induced significant CTL responses after one injection. Immunization of the monopalmitic acid-peptide conjugate intraperitoneally emulsified in Freund's complete adjuvant also induced a significant CTL response, but the magnitude was lower as compared to the intravenous route. The major advantages of the use of the simple monopalmitic acid-peptide conjugates are: (i) low costs of the fatty acid; (ii) coupling of lipid to peptide can be performed using the peptide synthesizer during standard peptide synthesis, and (iii) standard peptide m...Continue Reading

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Citations

Jul 20, 2002·The Lancet Infectious Diseases·Lbachir BenMohamedAnthony B Nesburn
Nov 24, 1999·The Journal of Peptide Research : Official Journal of the American Peptide Society·P HoogerhoutP J Roholl
Dec 14, 2011·International Journal of Microbiology·Gowrisankar RajamEdwin W Ades
Sep 8, 2017·Expert Review of Vaccines·Mathieu SurenaudJean-Daniel Lelièvre
Jun 19, 2021·Molecular Therapy Oncolytics·Dorian A StolkYvette van Kooyk

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