Monopolar gene electrotransfer enhances plasmid DNA delivery to skin.

Bioelectrochemistry
Anna BulyshevaRichard Heller

Abstract

A novel monopolar electroporation system and methodologies were developed for in vivo electroporation intended for potential clinical applications such as gene therapy. We hypothesized that an asymmetric anode/cathode electrode applicator geometry could produce favorable electric fields for electroporation, without the typical drawback associated with traditional needle and parallel plate geometries. Three monopolar electrode applicator prototypes were built and tested for gene delivery of reporter genes to the skin in a guinea pig model. Gene expression was evaluated in terms of kinetics over time and expression distribution within the treatment site. Different pulsing parameters, including pulse amplitude, pulse duration, and pulse number were evaluated. Monopolar gene electrotransfer significantly enhanced gene expression compared to controls over the course of 21 days. Gene expression distribution was observed throughout the full thickness of the epidermis, as well as notable expression in the deeper layers of the skin, including the dermis, and the underlying striated muscle without any damage at the treatment site, which is a substantial improvement over previously reported expression confined to the epidermis only. Expre...Continue Reading

References

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Apr 1, 2016·Current Gene Therapy·Amy DonateRichard Heller
Feb 6, 2018·Journal of Cardiovascular Electrophysiology·Adam WojtaszczykZdeněk Stárek

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Citations

Aug 10, 2021·Bioelectrochemistry·Carly BoyeAnna Bulysheva

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