Monosize poly(glycidyl methacrylate) beads for dye-affinity purification of lysozyme

International Journal of Biological Macromolecules
Evrim Banu Altintaş, Adil Denizli

Abstract

Cibacron Blue F3GA was covalently attached onto monosize poly(glycidyl methacrylate) [poly(GMA)] beads for purification of lysozyme from chicken egg white. Monosize poly(GMA) beads, 1.6 microm in diameter, were produced by a dispersion polymerization technique. The content of epoxy groups on the surface of the poly(GMA) sample determined by the HCl-pyridine method (3.8 mmol/g). Cibacron Blue F3GA loading was 1.73 mmol/g. The monosize beads were characterized by elemental analysis, FTIR and SEM. Adsorption studies were performed under different conditions in a batch system (i.e., medium pH, protein concentration, temperature and ionic strength). Maximum lysozyme adsorption amount of poly(GMA) and poly(GMA)-Cibacron Blue F3GA beads were 1.6 and 591.7 mg/g, respectively. The applicability of two kinetic models including pseudo-first order and pseudo-second order model was estimated on the basis of comparative analysis of the corresponding rate parameters, equilibrium adsorption capacity and correlation coefficients. Results suggest that chemisorption processes could be the rate-limiting step in the adsorption process. It was observed that after 10 adsorption-elution cycle, poly(GMA)-Cibacron Blue F3GA beads can be used without sig...Continue Reading

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Citations

May 11, 2012·Applied Biochemistry and Biotechnology·Deniz Aktaş UygunAdil Denizli
Sep 27, 2013·Applied Biochemistry and Biotechnology·Begüm AkdumanSinan Akgöl
Oct 31, 2013·Artificial Cells, Nanomedicine, and Biotechnology·Müge Andaç
May 29, 2008·Biomedical Chromatography : BMC·Ieva SutkeviciuteVladas-Algirdas Bumelis
Apr 4, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·María del Pilar FerrarisJorge A Rodríguez
Jan 3, 2015·Journal of Biomaterials Science. Polymer Edition·Murat UygunAdil Denizli
Aug 8, 2015·Materials Science & Engineering. C, Materials for Biological Applications·Duygu ÇimenAdil Denizli
Sep 13, 2008·Colloids and Surfaces. B, Biointerfaces·Nevra OztürkAdil Denizli
Oct 14, 2014·Applied Biochemistry and Biotechnology·Nalan TuzmenAdil Denizli
Jan 9, 2017·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Paula Chequer Gouveia MólLuis Antonio Minim
Jun 23, 2010·Journal of Biomaterials Science. Polymer Edition·Evrim Banu AltıntaşAdil Denizli
Nov 1, 2012·Artificial Cells, Nanomedicine, and Biotechnology·Kazım Köse, Adil Denizli
Sep 24, 2019·Journal of Biomaterials Science. Polymer Edition·Kamar HamadeAdil Denizli

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