Monosodium Urate Crystals Activate the Inflammasome in Primary Progressive Multiple Sclerosis

Frontiers in Immunology
Federica PianconeMario Clerici

Abstract

Inflammasome-driven inflammation is postulated to play a role in multiple sclerosis (MS), but there is no direct evidence that the nod-like receptor protein 3 (NLRP3) inflammasome is involved in MS pathogenesis. Uric acid was shown to be one of the "danger" signals involved in the activation of NLRP3 inflammasome; notably, the concentration of uric acid is increased in the serum and in the cerebrospinal fluid of MS individuals. To better investigate the role of the NLRP3 inflammasome in MS-associated inflammation, we primed with lipopolysaccharide and stimulated with monosodium urate crystals PBMCs of 41 MS patients with different disease phenotypes. Eleven individuals with primary progressive MS (PPMS), 10 individuals with stable relapsing-remitting MS (SMS), 10 individuals with acute relapsing-remitting MS (AMS), 10 individuals with benign MS were analyzed; 10 healthy controls were enrolled as well in the study. The expression of the NLRP3, apoptosis-associated speck-like protein containing CARD (ASC), caspase-1, caspase-8, IL-1β, and IL-18 inflammasome genes was evaluated by RT-PCR. NLRP3 and ASC-speck protein expression was analyzed by FlowSight AMNIS, whereas production of the pro-inflammatory cytokines IL-1β and IL-18 and...Continue Reading

References

Aug 1, 1991·Journal of the Neurological Sciences·N TsukadaK Yone
Jan 21, 1997·Proceedings of the National Academy of Sciences of the United States of America·K E BalashovH L Weiner
Nov 24, 1999·Journal of Neurology, Neurosurgery, and Psychiatry·R FurlanG Martino
Apr 4, 2001·Journal of Neurology·J DrulovićM Mostarica Stojković
Sep 12, 2001·Acta Neurologica Scandinavica·J Losy, A Niezgoda
Aug 6, 2002·Journal of Neuroimmunology·C L A MannC P Hawkins
Aug 13, 2002·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Ido YatsivPhilip F Stahel
Jan 6, 2004·Annals of Neurology·Barbara Cannella, Cedric S Raine
Oct 9, 2004·Multiple Sclerosis : Clinical and Laboratory Research·Wen-Xin HuangJan Hillert
Oct 6, 2005·Clinical Neurology and Neurosurgery·M RentzosD Vassilopoulos
Jan 13, 2006·Nature·Fabio MartinonJürg Tschopp
Jan 13, 2006·Nature·Sanjeev MariathasanVishva M Dixit
Oct 19, 2007·Multiple Sclerosis : Clinical and Laboratory Research·Fuhua PengZhong Pei
Jul 8, 2008·Nature Immunology·Annett HalleDouglas T Golenbock
Feb 17, 2009·American Journal of Respiratory and Critical Care Medicine·Pamela GasseIsabelle Couillin
Apr 4, 2009·Clinical Biochemistry·Angela M AmoriniGiuseppe Lazzarino
Jun 6, 2009·Clinical Chemistry and Laboratory Medicine : CCLM·Irena DujmovicJelena Drulovic
Jun 25, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Denis GrisJenny P-Y Ting
Jul 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Felix MeissnerArturo Zychlinsky
Dec 18, 2010·Trends in Molecular Medicine·Patrick J ShawThirumala-Devi Kanneganti
Dec 28, 2010·Current Pharmaceutical Design·P BossùG Scapigliati
Feb 23, 2011·Scandinavian Journal of Immunology·F Jadidi-Niaragh, A Mirshafiey
Mar 24, 2011·Expert Review of Clinical Immunology·Fumitaka SatoIkuo Tsunoda
Nov 19, 2011·Multiple Sclerosis International·Barbara TavazziGiuseppe Lazzarino
Jan 11, 2012·Journal of Neuroimmunology·Ying-Chun ChenQi Cheng
Feb 16, 2012·Neurological Research·Bo LiuJunjie Wei
Feb 1, 2013·Autoimmune Diseases·Makoto Inoue, Mari L Shinohara
Mar 27, 2013·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·Fereshteh AshtariArash Zahed
Aug 27, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Roberto PieriniThomas Henry
Oct 12, 2013·Frontiers in Immunology·Charles A DinarelloGilles Kaplanski
Feb 13, 2015·Journal of Neurology·Marcello MocciaVincenzo Brescia Morra
Jun 24, 2015·The Journal of Biological Chemistry·Christina AntonopoulosGeorge R Dubyak
Sep 17, 2015·European Journal of Neurology : the Official Journal of the European Federation of Neurological Societies·N GrigoriadisUNKNOWN ParadigMS Group
Jan 16, 2016·Mediators of Inflammation·Elisabetta VolpeGiovanna Borsellino
Feb 18, 2016·Acta biochimica Polonica·Sebastian WawrockiWieslawa Rudnicka
Jun 9, 2016·Journal of Neuroinflammation·Saskia Maria BurmJeffrey John Bajramovic
Jan 14, 2017·Scientific Reports·Tarcio Teodoro BragaNiels Olsen Saraiva Camara

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Citations

Aug 18, 2020·Cellular and Molecular Life Sciences : CMLS·C Corbin FryeHrishikesh S Kulkarni
Apr 28, 2020·Endocrine Practice : Official Journal of the American College of Endocrinology and the American Association of Clinical Endocrinologists·Ying WuZhongyan Shan
Aug 15, 2020·Molecular Oral Microbiology·Hye-Kyung JunBong-Kyu Choi
Oct 22, 2019·European Journal of Immunology·Andrea CossarizzaArturo Zychlinsky
Nov 30, 2019·Frontiers in Immunology·M Elizabeth DeerhakeMari L Shinohara
Nov 21, 2020·Neurology. Neuroimmunology and Neuroinflammation·Adil HarroudSergio E Baranzini
Oct 26, 2020·Basic & Clinical Pharmacology & Toxicology·Ali Saeedi-BoroujeniArash Alghasi
Jan 21, 2021·Endocrine Practice : Official Journal of the American College of Endocrinology and the American Association of Clinical Endocrinologists·Ying WuZhongyan Shan
Mar 7, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Federica PianconeMario Clerici
Jun 6, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Nadire Özenver, Thomas Efferth
Jul 24, 2021·Journal of Cellular and Molecular Medicine·Kun ChiQuan Hong
Jul 6, 2021·Annals of Neurology·Jan D LünemannManuel Comabella

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Methods Mentioned

BETA
PCR
ELISA

Software Mentioned

Flowsight AMNIS
IDEA
MedCalc
Flowsight
IDEAS

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