Monospecific monoclonal antibodies to keratin 1 carboxy terminal (synthetic peptide) and to keratin 10 as markers of epidermal differentiation

The British Journal of Dermatology
I M LeighE B Lane

Abstract

Monospecific antibodies to individual keratin polypeptides can be used to examine the tissue and cellular coexpression of members of keratin pairs. Monospecific monoclonal and polyclonal antibodies have been raised to keratins 1 and 10 using both crude cytoskeletal extracts and synthetic peptides. The tissue distribution of these keratins has been determined against a panel of freshly frozen normal tissues from humans, rodents and pigs. Epidermal expression has been examined in psoriatic plaques, and healing wounds, as examples of epidermal hyperproliferation. Cultured keratinocytes in monolayer (low calcium), stratified (high calcium), and complex cultures, transformed keratinocytes, and tumour cell lines, have been examined for the in vitro expression of these keratins. The sensitivity and precise localization of reactivity with these monospecific antibodies gives a highly accurate picture of individual cell expression. There is confirmation of coexpression of keratins 1 and 10 in epidermal and mucosal sites, and with keratin 16 in hyperproliferative states. These monospecific antibodies provide an important means of examining keratin expression in epidermal tumours and keratinizing disorders, and of seeking keratin mutations...Continue Reading

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Citations

Jan 1, 1995·Archives of Dermatological Research·C Lützow-HolmO P Clausen
Nov 1, 1994·European Journal of Cancer. Part B, Oral Oncology·G R OgdenE B Lane
Mar 31, 2004·Experimental Cell Research·Charlotte TrielUffe Birk Jensen
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Nov 28, 2013·PloS One·Christoforos TsantoulasRamin Raouf
Jul 13, 2012·Research in Veterinary Science·Sirin TheerawatanasirikulGunnaporn Suriyaphol
Feb 16, 2006·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Ichiro KurokawaAiro Tsubura
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Aug 16, 2017·Mediators of Inflammation·Andreas BayerJürgen Harder
Nov 25, 2000·Laboratory Investigation; a Journal of Technical Methods and Pathology·R M PorterE B Lane

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