PMID: 6991628Jun 1, 1980Paper

Monovalent fragments (Fab) of monoclonal antibodies to a sporozoite surface antigen (Pb44) protect mice against malarial infection

The Journal of Experimental Medicine
P PotocnjakVictor Nussenzweig

Abstract

Monoclonal antibodies (IG1, k) directed against a surface component of Plasmodium berghei sporozoites (Pb-44) confer complete protection to mice against a lethal inoculum of parasites. The degree of protection is a function of the number of parasites used in the challenge and of the antibody concentration in serum. Passive transfer of 10 micrograms of antibody per mouse abolished or profoundly diminished the infectivity of 10(3) sporozoites, but much higher amounts of antibody were required for complete protection against challenge with 10(4) parasites. Fab fragments of the monoclonal antibodies were as effective as the intact antibodies in mediating protection as determined by the neutralizing assay. This observation suggests that the antibodies interfere with a parasite function necessary for its infectivity, such as, for example, the ability to penetrate into the target cell or to multiply in the hepatocytes. When sporozoites are incubated with the intact monoclonal antibodies at 37 degrees C, a long filament appears at its posterior end (circumsporzoite precipitation [CSP] reaction). Fab fragments are ineffective at high concentrations. However, if after treatment with Fab, the sporozoites are incubated with rabbit antibodi...Continue Reading

Citations

Oct 1, 1984·European Journal of Clinical Microbiology·R A Polin
Dec 1, 1981·In Vitro·R H Kennett
Nov 1, 1984·Indian Journal of Pediatrics·K D MaudgilB S Narang
Jan 1, 1984·Transactions of the Royal Society of Tropical Medicine and Hygiene·P LelandM R Hollingdale
Jan 1, 1989·Transactions of the Royal Society of Tropical Medicine and Hygiene·J D Chulay
Jan 1, 1989·Transactions of the Royal Society of Tropical Medicine and Hygiene·G H LowellW T Hockmeyer
Dec 1, 1987·Immunology Letters·H R Gamble, K D Murrell
Mar 1, 1983·Molecular and Biochemical Parasitology·A N VermeulenJ H Meuwissen
Jun 1, 1985·Molecular and Biochemical Parasitology·J L Weber, W T Hockmeyer
Jan 1, 1985·Molecular and Biochemical Parasitology·A H CochraneR S Nussenzweig
May 1, 1986·Molecular and Biochemical Parasitology·A FerreiraV Nussenzweig
Sep 1, 1991·Molecular and Biochemical Parasitology·V SatchidanandamB Moss
Feb 1, 1995·Molecular and Biochemical Parasitology·B J SinaM Hollingdale
Dec 1, 1985·Parasitology Today·G A Targett, R E Sinden
Nov 2, 2013·Trends in Parasitology·Danielle I StanisicMichael F Good
Feb 28, 2001·Nature Biotechnology
Apr 1, 1988·Vaccine·L H Miller, M F Good

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.