PMID: 377287Apr 1, 1979Paper

Monovalent ionophores inhibit secretion of procollagen and fibronectin from cultured human fibroblasts

Proceedings of the National Academy of Sciences of the United States of America
N UchidaM L Tanzer

Abstract

Procollagen and fibronectin are major products of confluent fibroblasts in culture and both are released from the cells. Procollagen is secreted by known pathways, while the mechanism of fibronectin release is controversial. We find that the secretion of both these proteins can be reduced to 20% by low concentrations (0.1-1 muM) of ionophores that have affinity for monovalent cations. In contrast, little effect upon secretion was found for similar concentrations of an ionophore that binds divalent cations. Electron microscopy showed that the inhibition of secretion is accompanied by accumulation of membranous vacuoles. We believe that the ionophores impede secretion by acting on the secretory structures rather than on the proteins themselves. Biochemical studies supported this interpretation because no changes were detected in hydroxylation or glycosylation of procollagen or glycosylation of fibronectin, nor were significant changes in cellular amino acid incorporation observed. Pulse-chase studies indicated that the rates of secretion were impaired by the ionophore without enhancing intracellular degradation. The decreased secretory rates accounted for the lower levels of procollagen and fibronectin in the culture medium; no e...Continue Reading

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Citations

Jan 1, 1986·Archives of Virology·T KohamaA Sugiura
Nov 21, 1987·Biochimica Et Biophysica Acta·A BjørneboeC A Drevon
Dec 23, 1982·Clinica Chimica Acta; International Journal of Clinical Chemistry·W Jessup, R T Dean
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Sep 1, 1983·Cell Differentiation·M Galdieri, L Monaco
Jan 1, 1990·Comparative Immunology, Microbiology and Infectious Diseases·C ContiN Orsi
May 7, 1990·Biochimica Et Biophysica Acta·H H MollenhauerL D Rowe
Aug 1, 1985·Experimental Cell Research·G E JonesJ A Witkowski
May 3, 2002·European Journal of Biochemistry·Tomohiro SasanamiMasaru Toriyama
Feb 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·T F Bumol, R A Reisfeld
Mar 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·S JacobsP Cuatrecasas
Aug 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J PizzeyG Jones
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R PalM G Sarngadharan
Jan 1, 1991·The Journal of Cell Biology·R Bar-ShavitE Dejana
Dec 1, 1980·The Journal of Cell Biology·L KääriäinenK Penttinen
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Apr 28, 2012·American Journal of Physiology. Cell Physiology·Alexei SurguchevDhasakumar Navaratnam
Jun 1, 1989·The Journal of Membrane Biology·M F MarianiG D van Rossum
Oct 1, 1984·Experimental Cell Research·S Sai, M L Tanzer

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