Monoxenic liquid culture of the entomopathogenic nematode Steinernema carpocapsae using a culture medium containing whey kinetics and modeling

Journal of Biotechnology
Norberto Chavarría-HernándezAdriana-Inés Rodríguez-Hernández

Abstract

The submerged culture of the entomopathogenic nematode Steinernema carpocapsae and its symbiotic bacterium, Xenorhabdus nematophila, was carried out in orbitally agitated bottles using a culture medium containing whey (in grams per litre: 500 whey, 20 yeast extract, 10 dried egg yolk-food grade, 3 sodium chloride, 37 corn oil-food grade). Maximum total viable nematode concentrations of 198,333ml(-1) were achieved within fermentations of 24 days with 64% of the nematode population within the infective juvenile stage (IJ) (126,666ml(-1)) at the end. The kinetics of the bioprocess was well modelled using the four-parameter Sigmoidal model and the corresponding maximum specific rates of nematode production (0.47 day(-1)), carbohydrates consumption (0.0008g(carbohydrates)g(nematodes)(-1)day(-1)) and nitrogen consumption (4.44g(nitrogen)g(nematodes)(-1)day(-1)) are first proposed. Besides, X. nematophila appears to have the capacity of lactose hydrolysis.

Citations

Dec 13, 2007·Bioprocess and Biosystems Engineering·Norberto Chavarría-HernándezAdriana-Inés Rodríguez-Hernández
Jan 26, 2007·Biotechnology and Bioengineering·Norberto Chavarría-HernándezAdriana-Inés Rodríguez-Hernández
Jul 31, 2007·International Journal for Parasitology·Ernesto San-Blas, Simon R Gowen
Jul 28, 2020·Biotechnology and Bioengineering·Carlos Inocencio Cortés-Martínez, Norberto Chavarría-Hernández

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