Montagna symposium on epidermal stem cells oligonucleotide-directed gene correction in epidermis

The Journal of Investigative Dermatology. Symposium Proceedings
Kyonggeun Yoon

Abstract

Oligonucleotide-directed gene alteration produces a targeted DNA sequence change in the genome of mammalian cells. The advantage of this approach is that expression of the corrected gene is regulated in the same way as a normal gene. Reliable, sensitive, and standardized assays played a critical role in the measurement of gene correction frequency among different cell types and in evaluating the structure-activity relationship of oligonucleotides. Mechanistic studies using these assays have become critical for understanding the gene repair process and setting realistic expectations on the capability of this technology. The epidermis is an ideal tissue where oligonucleotides can be administered locally and the treated sites can be monitored easily. But given the low frequency of gene correction, general selection procedures and amplification of corrected cells via epidermal stem cells are ultimately needed to make the gene repair technology practical. Recent data suggest that the in vivo application of oligonucleotides may be capable of gene correction in epidermal stem cells and the subsequent expansion of the corrected cells may result in an apparent high-level and long-lasting gene repair. Advances in oligonucleotide delivery...Continue Reading

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Citations

Feb 28, 2019·Psychiatry Investigation·Jin-Won NohSo Hee Lee
Jul 7, 2005·Journal of Medical Internet Research·Kevin PatrickMarion F Zabinski
May 24, 2011·Journal of Educational Evaluation for Health Professions·Kasil OhMyung-Sook Ko

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