Monte Carlo Simulation of Low-Count Signals in Time-of-Flight Mass Spectrometry and Its Application to Single-Particle Detection

Analytical Chemistry
Alexander Gundlach-GrahamDetlef Günther

Abstract

Many modern time-of-flight mass spectrometry (TOFMS) instruments use fast analog-to-digital conversion (ADC) with high-speed digitizers to record mass spectra with extended dynamic range (compared to time-to-digital conversion). The extended dynamic range offered by ADC detection is critical for accurate measurement of transient events. However, the use of ADC also increases the variance of the measurements by sampling the gain statistics of electron multipliers (EMs) used for detection. The influence of gain statistics on the shape of TOF signal distributions is especially pronounced at low count rates and is a major contributor to measurement variance. Here, we use Monte Carlo methods to simulate low-ion-count TOFMS signals as a function of Poisson statistics and the measured pulse-height distribution (PHD) of the EM detection system. We find that a compound Poisson distribution calculated via Monte Carlo simulation effectively describes the shape of measured TOFMS signals. Additionally, we apply Monte Carlo simulation results to single-particle inductively coupled plasma (sp-ICP) TOFMS analysis. We demonstrate that subtraction of modeled TOFMS signals can be used to quantitatively uncover particle-signal distributions buried...Continue Reading

References

Jun 13, 1998·Analytical Chemistry·C S HoaglundD E Clemmer
Dec 3, 1998·Journal of the American Society for Mass Spectrometry·D C BarbacciM Van Stipdonk
May 5, 2000·Mass Spectrometry Reviews·M GuilhausV Mlynski
Dec 4, 2003·Journal of Mass Spectrometry : JMS·Michisato ToyodaItsuo Katakuse
Jan 15, 2009·Journal of Mass Spectrometry : JMS·Marvin L Vestal
May 1, 1979·Applied Optics·E H Eberhardt
Dec 15, 2010·Rapid Communications in Mass Spectrometry : RCM·Luca CappellinFlavia Gasperi
Oct 21, 2011·Environmental Toxicology and Chemistry·Denise M MitranoJames F Ranville
Dec 7, 2013·Analytical Chemistry·Francisco LabordaJavier Jiménez-Lamana
Jun 24, 2016·Analytical and Bioanalytical Chemistry·Manuel D MontañoJames F Ranville

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved