Monthly variations in ovine seminal plasma proteins analyzed by two-dimensional polyacrylamide gel electrophoresis

Theriogenology
J A CardozoJosé Alvaro Cebrián-Pérez

Abstract

This study was conducted to evaluate monthly changes in the ram seminal plasma protein profile using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) with a polyacrylamide linear gradient gel. Likewise, comparative analyses of the protein composition of ovine seminal plasma (SP) from ejaculates obtained along the year, and its relationship with sperm motility, viability and concentration of ejaculate were carried out. Western-blot analysis was performed to specifically detect P14, a ram SP protein postulated to be involved in sperm capacitation and gamete interaction [Barrios B, Fernández-Juan M, Muiño-Blanco T, Cebrián-Pérez JA. Immunocytochemical localization and biochemical characterization of two seminal plasma proteins which protect ram spermatozoa against cold-shock. J Androl 2005;26:539-49], and its variations along the year have also been established. The experiment was carried out from May 2003 to April 2004, with nine Rasa Aragonesa rams. Ejaculates obtained every 2 days were pooled and used for each assay, to avoid individual differences, and three two-dimensional SDS-PAGE gels were run for each month. The high resolution of the gradient gel allowed the image analysis software to detect around 252 protein...Continue Reading

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Citations

Mar 24, 2012·Animal : an International Journal of Animal Bioscience·J J RobinsonL M Williams
Sep 7, 2012·Reproduction, Fertility, and Development·N S JuyenaC Stelletta
Jun 5, 2012·Cell and Tissue Banking·Mariana RovegnoMayra Elena Ortiz D'Avila Assumpção
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Aug 3, 2021·Frontiers in Cell and Developmental Biology·Mari-Carmen Fuentes-AlberoFrancisco Alberto García-Vázquez

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