More sensitive and simpler immune complex transfer enzyme immunoassay for antithyroglobulin IgG in serum

Journal of Clinical Laboratory Analysis
T KohnoE Ishikawa

Abstract

A more sensitive and simpler immune complex transfer enzyme immunoassay for antithyroglobulin IgG in serum and its use for the measurement of antithyroglobulin IgG in healthy subjects and patients with thyroid diseases are described. Antithyroglobulin IgG in test serum was reacted simultaneously with dinitrophenylthyroglobulin and thyroglobulin-beta-D-galactosidase conjugate. The complex formed of antithyroglobulin IgG, dinitrophenyl thyroglobulin, and thyroglobulin-beta-D-galactosidase conjugate was trapped onto two polystyrene balls coated with affinity-purified rabbit (antidinitrophenyl bovine serum albumin) IgG. The polystyrene balls were washed to eliminate nonspecific IgG in the test serum, and the complex was eluted from the polystyrene balls with dinitrophenyl-L-lysine and transferred to two polystyrene balls coated with affinity-purified rabbit (anti-human IgG gamma-chain) IgG. beta-D-Galactosidase activity bound to the polystyrene balls was assayed by fluorimetry using 4-methylumbelliferyl-beta-D-galactoside as substrate. As compared with the previous immune complex transfer enzyme immunoassay, the procedure was simplified by reducing one incubation step and one washing step, and the detection limit of antithyroglobul...Continue Reading

Citations

Dec 17, 1990·Clinica Chimica Acta; International Journal of Clinical Chemistry·E IshikawaK Hirota
Jan 1, 1989·Journal of Clinical Laboratory Analysis·E Ishikawa, T Kohno
Jul 21, 1998·Annals of the New York Academy of Sciences·M HayashiK Kida

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