More than just fibers: an aqueous method for the production of innovative recombinant spider silk protein materials

Biomacromolecules
Justin A JonesRandolph V Lewis

Abstract

Spider silk is a striking and robust natural material that has an unrivaled combination of strength and elasticity. There are two major problems in creating materials from recombinant spider silk proteins (rSSps): expressing sufficient quantities of the large, highly repetitive proteins and solvating the naturally self-assembling proteins once produced. To address the second problem, we have developed a method to rapidly dissolve rSSps in water in lieu of traditional organic solvents and accomplish nearly 100% solvation and recovery of the protein. Our method involves generating pressure and temperature in a sealed vial by using short, repetitive bursts from a conventional microwave. The method is scalable and has been successful with all rSSps used to date. From these easily generated aqueous solutions of rSSps, a wide variety of materials have been produced. Production of fibers, films, hydrogels, lyogels, sponges, and adhesives and studies of their mechanical and structural properties are reported. To our knowledge, ours is the only method that is cost-effective and scalable for mass production. This solvation method allows a choice of the physical form of product to take advantage of spider silks' mechanical properties with...Continue Reading

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Citations

Sep 13, 2015·Applied Microbiology and Biotechnology·Elena DoblhoferThomas Scheibel
Apr 12, 2016·ACS Biomaterials Science & Engineering·Cameron G CopelandRandolph V Lewis
Mar 5, 2015·Saudi Journal of Biological Sciences·Faisal Hammad Mekky KouaAkio Tani
Nov 26, 2016·International Journal of Molecular Sciences·Justin A JonesRandolph V Lewis
Aug 19, 2017·Bioinspiration & Biomimetics·Jonas O WolffSean J Blamires
Aug 15, 2017·Macromolecular Bioscience·Christopher ThammThomas Scheibel
May 22, 2018·Biotechnology Journal·Ke Zheng, Shengjie Ling
Feb 3, 2018·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Martin HumenikThomas Scheibel
Aug 17, 2018·Scientific Reports·Richard E DeckerJustin A Jones
Mar 23, 2021·Protein Expression and Purification·Gargi BhattacharyyaJustin A Jones
Apr 17, 2021·Acta Biomaterialia·Yoonjung KimSungsoo Na
Oct 15, 2020·Trends in Biotechnology·Dominic R WhittallEriko Takano
Mar 13, 2017·ACS Biomaterials Science & Engineering·Andreas Koeppel, Chris Holland
Aug 12, 2019·ACS Biomaterials Science & Engineering·Thomas I HarrisElizabeth Vargis

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