PMID: 15329271Aug 27, 2004Paper

Morphine-induced late cardioprotection: potential role of inducible nitric oxide synthase

Zhonghua yi xue za zhi
Enyi ShiYoshiki Nakajima

Abstract

To explore the late cardioprotection induced by morphine preconditioning and determine the role of inducible nitric oxide synthase (iNOS) in mediating this effect. Thirty-two wild type (WT) mice and 16 iNOS gene knockout mice, totally 48 mice, underwent ligation of the left anterior descending coronary artery (LAD) for 45 minutes and reperfusion for 120 minutes. The 32 wild type mice were randomly divided into 4 groups of 8 mice: WT control group, 24 hours before the heart occlusion, normal saline was given; WT + morphine group, 24 hours before the heart occlusion morphine was administered; WT + SMT group, 24 hours before the heart occlusion normal saline was administered and 30 minutes before heart occlusion S-methylthiourea sulfate (SMT), a selective inhibitor of iNOS, was administered; and WT + morphine + SMT group, 24 hours before the heart occlusion morphine was administered and 30 minutes before heart occlusion SMT was administered. The 16 iNOS gene knockout mice were randomly divided into 2 groups of 8 mice: iNOS (-/-) control group (24 hours before the heart occlusion normal saline was given) and iNOS (-/-) + morphine group (24 hours before heart occlusion. morphine was administered). One hundred and twenty minutes afte...Continue Reading

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