Morphine-induced reciprocal alterations in G alpha s and opioid peptide mRNA levels in discrete brain regions

Journal of Neuroscience Research
R Basheer, A Tempel


The mechanisms involved in the development of morphine tolerance and dependence are still unknown. Recently much attention has been directed toward the changes in post receptor events. Opiate receptors, like other hormone and neurotransmitter receptors, have been shown to mediate their effects through guanine nucleotide binding proteins (G-proteins). This, in turn, may cause alterations in intracellular events, one of which is transcription of specific genes. We investigated the changes in the levels of mRNA of proenkephalin (PPE) and prodynorphin (DYN) and the stimulatory G protein alpha subunit (G alpha s) in adult morphine tolerant rats. Chronic morphine treatment induced reciprocal alterations in the levels of opioid peptide mRNA and G alpha s mRNA in discrete brain regions. In striatum, PPE mRNA decreased by 49% (P < .01) and in hypothalamus, DYN mRNA showed a decrease of 21% (P < .01). In contrast, G alpha s mRNA increased 20% (P < .01) in striatum and 97% (P < .01), in hypothalamus. In hippocampus the changes were reversed: PPE mRNA increased (55%, P < .05) and G alpha s mRNA decreased (33%, P < .01). Frontal cortex exhibited a small decrease in PPE (11.5%, P < .05) without any change on G alpha s or DYN mRNA levels. The...Continue Reading


Jun 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·R A NicollR Guillemin
Apr 1, 1976·Biochemical Pharmacology·C B Pert, S H Snyder
Feb 1, 1990·Trends in Pharmacological Sciences·S M Crain, K F Shen
Jan 9, 1989·Brain Research·E J NestlerJ F Tallman
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·L L WerlingB M Cox
Apr 1, 1988·Neuroscience·J M PalaciosW Lichtensteiger
Jul 15, 1986·The Journal of Comparative Neurology·J H Fallon, F M Leslie
Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·A Tempel, R S Zukin
May 1, 1988·DNA·P E DanielsonJ G Sutcliffe
Apr 1, 1988·The Journal of Physiology·D V Madison, R A Nicoll
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·O CivelliE Herbert
Mar 1, 1974·Nature·W A Klee, R A Streaty
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·F TangJ P Schwartz
Aug 25, 1983·Nucleic Acids Research·R J Milner, J G Sutcliffe
Feb 1, 1983·Pharmacology, Biochemistry, and Behavior·W A Corrigall
Nov 15, 1982·Life Sciences·R F RitzmannJ Z Fields


Jul 1, 1996·Physiology & Behavior·S E CardenM A Hofer
Nov 1, 1995·Brain Research. Molecular Brain Research·A TempelR Basheer
Jun 24, 1998·Brain Research. Molecular Brain Research·Y FukunagaH Yamamoto
May 14, 2004·The Journal of Peptide Research : Official Journal of the American Peptide Society·R SlamberováI Vathy
Oct 7, 2004·European Journal of Pharmacology·Ryszard Przewlocki
Oct 1, 2009·Physiological Reviews·Julie Le MerrerBrigitte L Kieffer
Mar 23, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sabine SpijkerAugust B Smit
Apr 30, 1999·Journal of Psychopharmacology·G DarcourtJ Lavoisy
Dec 7, 2018·Nature Reviews. Neuroscience·Cherkaouia KibalyPing-Yee Law
Sep 1, 1995·The American Journal of Physiology·D Borsook, S E Hyman

Related Concepts

Preproenkephalin B
Brain Chemistry
Drug Tolerance
Dynorphin (1-17)
Organum Vasculosum Laminae Terminalis
Morphine Sulfate (2: 1), Pentahydrate
Protein Precursors
Poly(A) Tail
Northern Blot

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Synthetic Genetic Array Analysis

Synthetic genetic arrays allow the systematic examination of genetic interactions. Here is the latest research focusing on synthetic genetic arrays and their analyses.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Computational Methods for Protein Structures

Computational methods employing machine learning algorithms are powerful tools that can be used to predict the effect of mutations on protein structure. This is important in neurodegenerative disorders, where some mutations can cause the formation of toxic protein aggregations. This feed follows the latests insights into the relationships between mutation and protein structure leading to better understanding of disease.

Congenital Hyperinsulinism

Congenital hyperinsulinism is caused by genetic mutations resulting in excess insulin secretion from beta cells of the pancreas. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Epigenetic Memory

Epigenetic memory refers to the heritable genetic changes that are not explained by the DNA sequence. Find the latest research on epigenetic memory here.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Femoral Neoplasms

Femoral Neoplasms are bone tumors that arise in the femur. Discover the latest research on femoral neoplasms here.