PMID: 9429908Jan 16, 1998Paper

Morphine induces splenocyte apoptosis and enhanced mRNA expression of cathepsin-B

Inflammation
P C SinghalN Gibbons

Abstract

Morphine has been demonstrated to modulate immune function. We studied whether morphine modulates apoptosis of splenocytes. Splenocytes were isolated from control and morphine treated rats. Splenocytes isolated from morphine treated rats showed increased percentage (P < 0.001) of apoptosis when compared to splenocytes isolated from untreated rats (control, 4.7 +/- 1.0% apoptotic splenocytes/field vs. morphine, 47.8 +/- 3.4% apoptotic splenocytes/field). These results were further confirmed by gel electrophoresis as well as by end-labeling DNA of splenocytes isolated from control and morphine treated rats. Splenocytes from morphine treated rats showed a classical ladder pattern with integer multiples of 180 base pairs. Splenocytes from morphine treated rats also showed increased mRNA expression of cathepsin-B, a gene associated with active cell death. These results suggest that morphine may also be modulating immune function by enhancing apoptosis of splenocytes.

Citations

Sep 24, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jinghua WangSabita Roy
Jul 23, 2016·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·A Carmona-BayonasJ A Virizuela
Jun 26, 2004·The European Journal of Neuroscience·Valeriya K KhurdayanKurt F Hauser
Mar 25, 2004·Journal of Leukocyte Biology·Rajani S BhatPravin C Singhal
Mar 20, 2008·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Yan ZhangLong-Chuan Yu
Oct 24, 2002·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Avi NathJadwiga T Turchan

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