Morphine mimics the antiapoptotic effect of preconditioning via an Ins(1,4,5)P3 signaling pathway in rat ventricular myocytes

American Journal of Physiology. Heart and Circulatory Physiology
Stéphanie Barrère-LemaireChristophe Piot

Abstract

Morphine has cardioprotective effects against ischemic-reperfusion injuries. This study investigates whether morphine could mimic the antiapoptotic effect of preconditioning using a model of cultured neonatal rat cardiomyocytes subjected to metabolic inhibition (MI). To quantify MI-induced apoptosis, DNA fragmentation and mitochondrial cytochrome c release levels were measured by ELISA. MI-dependent DNA fragmentation was prevented by both Z-VAD-fmk (20 microM), a pan-caspase inhibitor, and cyclosporine A (CsA; 5 microM), a mitochondrial pore transition blocker, added during MI (36% and 54% decrease, respectively). MI-dependent cytochrome c release was not blocked by Z-VAD-fmk but was decreased (38%) by CsA during MI. Metabolic preconditioning (MIP) and preconditioning with morphine (1 microM) were also assessed. MI-dependent DNA fragmentation and cytochrome c release were prevented by MIP (40% and 45% decrease, respectively) and morphine (34% and 45%, respectively). The antiapoptotic effect of morphine was abolished by naloxone (10 nM), a nonselective opioid receptor antagonist, or xestospongin C (XeC, 400 nM), an inhibitor of inositol (1,4,5)-trisphosphate [Ins(1,4,5)P(3)]-mediated Ca(2+) release. Ca(2+) preconditioning, induc...Continue Reading

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Citations

Jun 3, 2014·BMC Pharmacology & Toxicology·Lisa Drange HoleJan Schjøtt
Mar 2, 2010·The Journal of Surgical Research·Linghai LiBingxi Zhang
Sep 16, 2006·Peptides·Richard J Bodnar, Gad E Klein
Mar 20, 2008·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Yan ZhangLong-Chuan Yu
Sep 27, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Karin PrzyklenkPeter Whittaker
May 30, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Karin PrzyklenkPeter Whittaker
May 21, 2016·Medicinal Research Reviews·Leonid N MaslovJames M Downey
Oct 4, 2005·Cellular Signalling·Damien S K Samways, Graeme Henderson

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