Morphine suppresses IFN signaling pathway and enhances AIDS virus infection.

PloS One
Yizhong WangWen-Zhe Ho

Abstract

Opioids exert a profound influence on immunomodulation and enhance HIV infection and replication. However, the mechanism(s) of their action remains to be determined. We thus investigated the impact of morphine on the intracellular innate antiviral immunity. Seven-day-cultured macrophages were infected with equal amounts of cell-free HIV Bal or SIV Delta(B670) for 2 h at 37°C after 24 h of treatment with or without morphine. Effect of morphine on HIV/SIV infection and replication was evaluated by HIV/SIV RT activity assay and indirect immunofluorescence for HIV p24 or SIV p28 antigen. The mRNA expression of cellular factors suppressed or induced by morphine treatment was analyzed by the real-time RT-PCR. We demonstrated that morphine treatment of human blood monocyte-derived macrophages significantly inhibited the expression of interferons (IFN-α, IFN-β and IFN-λ) and IFN-inducible genes (APOBEC3C/3F/3G and 3H). The further experiments showed that morphine suppressed the expression of several key elements (RIG-I and IRF-7) in IFN signaling pathway. In addition, morphine treatment induced the expression of suppressor of cytokine signaling protein-1, 2, 3 (SOCS-1, 2, 3) and protein inhibitors of activated STAT-1, 3, X, Y (PIAS-1, ...Continue Reading

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Citations

Sep 30, 2014·Peptides·Richard J Bodnar
Nov 20, 2015·Frontiers in Microbiology·Xu WangWen-Zhe Ho
Oct 13, 2015·Experimental Gerontology·Yenisel Cruz-AlmeidaJoseph L Riley
Aug 21, 2012·Microbiology and Immunology·Esaki Muthu ShankarNegar Shafiei Sabet
Jan 29, 2014·JAK-STAT·Melanie Busch-Dienstfertig, Sara González-Rodríguez
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Jul 16, 2016·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Bingyu LiangLi Ye
Oct 3, 2014·PloS One·Mohamed Abdel-MohsenSatish K Pillai
Nov 5, 2019·Frontiers in Immunology·Aniella MurphyJoan W Berman
Sep 26, 2020·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Livio AzzoniLuis J Montaner
Dec 6, 2019·Frontiers in Immunology·Xu WangWen-Zhe Ho
Oct 9, 2020·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Shilpa BuchAshutosh Tripathi
Jun 2, 2021·Bulletin of Mathematical Biology·Naveen K Vaidya, Michael Peter
May 20, 2020·Diabetes & Metabolic Syndrome·Neda RoshanravanMehdi Hedayati

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Methods Mentioned

BETA
PCR
fluorescence microscopy

Software Mentioned

MyiQ
SPSS

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