Morpho-functional modifications of human syncytiotrophoblast plasma membrane during pregnancy induced hypertension

Molecular and Cellular Biochemistry
G BiaginiL Mazzanti

Abstract

A decrease of Na+/K(+)-ATPase activity has been reported in syncytiotrophoblast plasma membrane (SPM) obtained from pregnancy induced hypertensive (PIH) women. The aim of the present work was to verify if the reported modifications in activity are due to a decreased number of enzymatic molecules or to a conformational change of the enzyme itself. Morphological studies were performed in order to better understand the relations between the enzymatic protein and the lipid bilayer. Kinetic studies were also performed. SPM obtained from PIH showed: i) an increased affinity of Na+/K(+)-ATPase for ouabain binding, ii) a significant change in the maximum velocity of the enzyme, iii) a higher distribution factor (DF) of intramembrane particles (IMPs) in the exoplasmic face of the membrane, iv) a decreased mean diameter of IMPs both in the protoplasmic and exoplasmic faces, v) a decreased number of IMPs in the exoplasmic face. In conclusion, a conformational modification seems to be at the basis of the decreased Na+/K(+)-ATPase activity during PIH as suggested by binding, ultrastructural and kinetic data herein reported.

References

Oct 3, 1975·Science·D BrantonR S Weinstein
Apr 1, 1991·Experimental and Molecular Pathology·C RomaniniL Mazzanti
Jul 1, 1990·Diabetes·L MazzantiR De Pirro
Jan 29, 1990·Biochimica Et Biophysica Acta·Y Kudo, C A Boyd
Jan 1, 1986·The Journal of Membrane Biology·N P Illsley, A S Verkman
Feb 1, 1989·European Journal of Clinical Investigation·L MazzantiE Bertoli
Jan 1, 1988·Methods in Enzymology·J C Skou
Jul 1, 1986·Physiological Reviews·T Clausen
Feb 1, 1987·The American Journal of Physiology·J W Bowen, A McDonough
Feb 25, 1988·The New England Journal of Medicine·R C Goodlin
Apr 1, 1988·American Journal of Obstetrics and Gynecology·D A Davey, I MacGillivray
Sep 9, 1985·Biochimica Et Biophysica Acta·D L Yudilevich, J H Sweiry
Sep 1, 1983·The Journal of Cell Biology·D S RoosR L Davidson
Jan 30, 1981·The Journal of Membrane Biology·J M BissonnetteK M Acott
Jun 1, 1981·The Journal of Physiology·C A Boyd, E K Lund

❮ Previous
Next ❯

Citations

Jan 20, 1999·European Journal of Clinical Investigation·N CesterL Mazzanti

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.