Morphogenesis and differentiation of grafted blastemas formed in vitro from amputated hindlimbs of larval Xenopus laevis

The Journal of Experimental Zoology
S BernardiniS Filoni

Abstract

The present study was designed to test the morphogenetic potency of limb blastemas formed in vitro from amputated limbs of larval Xenopus laevis. Hindlimbs of larvae at stage 55 (according to Nieuwkoop and Faber [1956] Normal Table of Xenopus laevis (Daudin)) were amputated through the tarsalia, excised at the base of the thigh and cultured in Leibovitz's L-15 supplemented with 2% FCS. After 8-10 days, 50% of the cultured limbs formed a conic blastema on the amputation surface. However, on the excision surface no blastema was present. Three different parts (blastema, blastema with the shank region and proximal part of the limb) of the cultured limbs were then grafted to the axial musculature or to the hindlimb of stage 57 host larvae. Results showed that the blastema formed in vitro were true autodifferentiating regeneration blastemas, since they were able to form well-differentiated autopodia not only when grafted with the shank region to a neutral territory (axial musculature) or to the limb territory, but also when transplanted alone to the two environments. The morphological complexity (no. of toes) of the autopodia differentiated from the grafted blastemas was superimposable to that observed in vivo. Moreover, as in vivo, ...Continue Reading

References

Jul 1, 1992·The Journal of Experimental Zoology·S M CannataS Filoni
Nov 1, 1991·The Journal of Experimental Zoology·S FiloniS M Cannata
Mar 1, 1990·Differentiation; Research in Biological Diversity·S Filoni, L Paglialunga
Jan 1, 1984·Differentiation; Research in Biological Diversity·D L Stocum
Sep 1, 1970·Wilhelm Roux' Archiv Für Entwicklungsmechanik Der Organismen·N J de Both

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