Morphokinetics and in vitro developmental potential of monopronucleated ICSI zygotes until the blastocyst stage

Zygote : the Biology of Gametes and Early Embryos
Silvia MateoMontserrat Boada

Abstract

The aim of this study was to provide a more comprehensive understanding of 1PN intracytoplasmic sperm injection (ICSI) zygotes. To achieve this objective, we assessed whether all 1PN-derived embryos showed a similar morphokinetic pattern, and if the morphokinetic behaviour of 1PN-derived embryos was comparable with that of 2PN-derived embryos. In total, 149 1PN ICSI zygotes (study group) and 195 2PN ICSI zygotes (control group) were included in the study. Embryo development potential was evaluated in terms of blastocyst rate. Morphokinetic parameters, including the pronucleus diameter and kinetics of in vitro development, were also analyzed. Embryos derived from 1PN ICSI zygotes showed impaired development compared with 2PN-derived embryos, with blastocyst rates of 28.9% and 67.2%, respectively. The diameter of the pronucleus of 1PN zygotes was larger than that of 2PN zygotes. When compared with 2PN-derived embryos, those derived from 1PN zygotes had a visible pronucleus for a shorter time, in addition to a longer syngamy time and slower kinetic behaviour from two to nine cells. When 1PN-derived blastocysts and 2PN-derived blastocysts were compared, the developmental kinetics were similar in both groups, except for a delayed an...Continue Reading

References

Mar 1, 1995·Biology of Reproduction·J LevronJ Cohen
Sep 12, 2000·Human Genetics·A S LimS L Yu
Nov 7, 2009·Journal of Assisted Reproduction and Genetics·Hongqing LiaoGuangxiu Lu
Jul 26, 2011·Placenta·Markus MontagMaria Köster
Aug 11, 2011·Human Reproduction·Marcos MeseguerJose Remohí
Aug 11, 2012·Reproductive Biomedicine Online·María CruzMarcos Meseguer
Aug 29, 2012·Fertility and Sterility·Irene RubioMarcos Meseguer
Mar 23, 2013·Reproductive Biomedicine Online·Alison CampbellCristina Fontes Lindemann Hickman
Jan 25, 2014·Journal of Assisted Reproduction and Genetics·Mette Warming JoergensenKirstine Kirkegaard
Mar 4, 2014·Reproductive Biomedicine Online·Jesús AguilarMarcos Meseguer
Feb 19, 2015·Journal of Assisted Reproduction and Genetics·Robert MilewskiWaldemar Kuczyński
Jun 27, 2015·Journal of Assisted Reproduction and Genetics·Fumiaki ItoiYasutaka Murata
Sep 24, 2015·Journal of Assisted Reproduction and Genetics·Yoshiteru KaiYasuyuki Mio
Oct 11, 2015·Fertility and Sterility·Francisca MartinezBuenaventura Coroleu
Dec 13, 2016·Reproductive Biomedicine Online·C LagallaA Borini
May 13, 2017·Journal of Assisted Reproduction and Genetics·S MateoM Boada
Aug 23, 2017·Developmental Cell·David JukamJan M Skotheim
Aug 31, 2017·Scientific Reports·Christian S OttoliniAlan H Handyside
Jan 5, 2016·Reproductive Medicine and Biology·Kyoko Iwata, Yasuyuki Mio
Feb 28, 2019·Zygote : the Biology of Gametes and Early Embryos·Gemma FabozziClaudio Piscitelli

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.