PMID: 9435073Jan 22, 1998Paper

Morpholine degradation pathway of Mycobacterium aurum MO1: direct evidence of intermediates by in situ 1H nuclear magnetic resonance

Applied and Environmental Microbiology
Bruno CombourieuN Truffaut

Abstract

Resting Mycobacterium aurum MO1 cells were incubated with morpholine, a waste from the chemical industry. The kinetics of biodegradation was monitored by using in situ nuclear magnetic resonance (NMR). The incubation medium was directly analyzed by 1H NMR. This technique allowed the unambiguous identification of two intermediates of the metabolic pathway involved in the biodegradation process, glycolate and 2-(2-aminoethoxy)acetate. The latter compound, which was not commercially available, was synthesized, in three steps, from 2-(2-aminoethoxy)ethanol. Quantitative analysis of the kinetics of degradation of morpholine was performed by integrating the signals of the different metabolites in 1H-NMR spectra. Morpholine was degraded within 10 h. The intermediates increased during the first 10 h and finally disappeared after 20 h incubation. Assays of degradation were also carried out with glycolate and ethanolamine, hypothetical intermediates of the morpholine degradation pathway. They were degraded within 4 and 8 h, respectively. Until now, no tool for direct detection of intermediates or even morpholine has been available, consequently, only hypothetical pathways have been proposed. The approach described here gives both qualita...Continue Reading

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Citations

Nov 27, 2002·Applied and Environmental Microbiology·Nicolas HarouneAnne-Marie Delort
Aug 2, 2003·Emerging Infectious Diseases·Joseph O Falkinham
Sep 29, 2004·FEMS Microbiology Letters·Talaat El SebaiFabrice Martin-Laurent
Feb 10, 2009·Applied and Environmental Microbiology·Joseph O Falkinham
Jan 22, 1998·Applied and Environmental Microbiology·P PoupinA M Delort

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