Morpholino-Mediated Knockdown of Ciliary Genes in Euplotes vannus , a Novel Marine Ciliated Model Organism

Frontiers in Microbiology
Danxu TangChengtian Zhao

Abstract

Cilia are highly conserved organelles present in almost all types of eukaryotic cells, and defects in cilia structure and/or function are related to many human genetic disorders. Single-celled ciliated protists, which possess diverse types of cilia, are remarkable model organisms for studying cilia structures and functions. Euplotes vannus is a representative ciliate with many intriguing features; for example, it possesses extensively fragmented somatic genomes and a high frequency of + 1 programmed ribosomal frameshifting. However, the molecular mechanisms underlying these remarkable traits remain largely unknown, mainly due to the lack of efficient genetic manipulation tools. Here, we describe the first application of a morpholino-based strategy to knockdown gene expression in E. vannus. Through interfering with the function of two ciliary genes, ZMYND10 and C21ORF59, we showed that these two genes are essential for the ciliary motility and proliferation of E. vannus cells. Strikingly, both ZMYND10- and C21ORF59-knockdown cells developed shorter cilia in the ventral cirri, a special type of ciliary tuft, suggesting a novel role for these genes in the regulation of cilia length. Our data provide a new method to explore gene fu...Continue Reading

References

Mar 1, 1991·The Journal of protozoology·S E Baird, L A Klobutcher
Jun 1, 1997·Antisense & Nucleic Acid Drug Development·J Summerton, D Weller
Oct 3, 2006·Annual Review of Physiology·Peter Satir, Søren Tvorup Christensen
Apr 22, 2011·The New England Journal of Medicine·Friedhelm HildebrandtNicholas Katsanis
Nov 9, 2012·The Journal of Eukaryotic Microbiology·John A Kloetzel, Terrence W Brann
Jul 23, 2013·The Journal of Eukaryotic Microbiology·Xuming PanWeibo Song
Oct 8, 2013·American Journal of Human Genetics·Christina Austin-TseFriedhelm Hildebrandt
May 9, 2014·Nature·Deepankar Pratap SinghEric Meyer
Oct 8, 2014·Current Biology : CB·Eric R Brooks, John B Wallingford
Mar 30, 2016·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Zhu SongChengtian Zhao
Nov 22, 2016·Nature Structural & Molecular Biology·Alexei V LobanovVadim N Gladyshev
Apr 22, 2017·European Journal of Protistology·John C Clamp, Denis H Lynn
Oct 29, 2017·RNA·Jaspreet S KhuranaLaura F Landweber

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Methods Mentioned

BETA
antisense oligonucleotide
antisense oligonucleotides
scanning electron microscopy
PCR

Software Mentioned

Prism
Image J

Related Concepts

Related Feeds

Basal Ganglia

Basal Ganglia are a group of subcortical nuclei in the brain associated with control of voluntary motor movements, procedural and habit learning, emotion, and cognition. Here is the latest research.