Abstract
Cilia are highly conserved organelles present in almost all types of eukaryotic cells, and defects in cilia structure and/or function are related to many human genetic disorders. Single-celled ciliated protists, which possess diverse types of cilia, are remarkable model organisms for studying cilia structures and functions. Euplotes vannus is a representative ciliate with many intriguing features; for example, it possesses extensively fragmented somatic genomes and a high frequency of + 1 programmed ribosomal frameshifting. However, the molecular mechanisms underlying these remarkable traits remain largely unknown, mainly due to the lack of efficient genetic manipulation tools. Here, we describe the first application of a morpholino-based strategy to knockdown gene expression in E. vannus. Through interfering with the function of two ciliary genes, ZMYND10 and C21ORF59, we showed that these two genes are essential for the ciliary motility and proliferation of E. vannus cells. Strikingly, both ZMYND10- and C21ORF59-knockdown cells developed shorter cilia in the ventral cirri, a special type of ciliary tuft, suggesting a novel role for these genes in the regulation of cilia length. Our data provide a new method to explore gene fu...Continue Reading
References
Mar 1, 1991·The Journal of protozoology·S E Baird, L A Klobutcher
Jun 1, 1997·Antisense & Nucleic Acid Drug Development·J Summerton, D Weller
Jun 2, 2001·Genome Biology·D R Corey, J M Abrams
Oct 3, 2006·Annual Review of Physiology·Peter Satir, Søren Tvorup Christensen
Apr 22, 2011·The New England Journal of Medicine·Friedhelm HildebrandtNicholas Katsanis
Nov 9, 2012·The Journal of Eukaryotic Microbiology·John A Kloetzel, Terrence W Brann
Feb 6, 2013·PLoS Biology·Estienne C SwartLaura F Landweber
Jul 23, 2013·The Journal of Eukaryotic Microbiology·Xuming PanWeibo Song
Oct 8, 2013·American Journal of Human Genetics·Christina Austin-TseFriedhelm Hildebrandt
May 9, 2014·Nature·Deepankar Pratap SinghEric Meyer
Oct 8, 2014·Current Biology : CB·Eric R Brooks, John B Wallingford
Mar 30, 2016·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Zhu SongChengtian Zhao
Jul 19, 2016·Cell·Estienne Carl SwartMariusz Nowacki
Nov 22, 2016·Nature Structural & Molecular Biology·Alexei V LobanovVadim N Gladyshev
Apr 22, 2017·European Journal of Protistology·John C Clamp, Denis H Lynn
Oct 29, 2017·RNA·Jaspreet S KhuranaLaura F Landweber
Jun 13, 2018·Genes & Genomics·Bo-Mi KimYoung-Mi Lee
Nov 14, 2018·Nature Genetics·Xiaoli ZhangChengtian Zhao
Dec 20, 2018·Cell Cycle·Yaohan JiangFeng Gao
Apr 16, 2019·Molecular Ecology Resources·Xiao ChenWeibo Song