Abstract
Porcine reproductive and respiratory syndrome (PRRS) causes extensive economic losses in the swine industry. Current strategies and vaccines to control the disease are inadequate. We previously demonstrated that peptide-conjugated phosphorodiamidate morpholino oligomers (PPMOs) could potently inhibit PRRS virus (PRRSV) replication in cell cultures. PPMOs are single-stranded DNA analogues containing a modified backbone and cell-penetrating peptide. PPMOs are nuclease-resistant, water-soluble, can enter cells readily and exhibit highly specific binding to complementary RNA. In this study, we examined PPMO-mediated inhibition of PRRSV replication in a primary culture of porcine pulmonary alveolar macrophages (PAMs). PAMs were collected from piglets, pre-incubated in culture and infected with PRRSV. Viability, cytopathic effects, virus yield and apoptosis of PAMs in the presence or absence of a PPMO (5UP2) were examined. The 5UP2 PPMO is complementary to a conserved sequence in the 5'-terminal region of the PRRSV genome. The level of several interferon-associated gene products and activity of caspases were monitored. PRRSV infection induced the activity of caspases-3/7, -8 and -9 significantly. Treatment of PAMs with 5UP2 resulted ...Continue Reading
Citations
Aug 27, 2010·Journal of Virology·Deendayal PatelYan-Jin Zhang
Mar 2, 2013·Journal of Virology·Rong WangYan-Jin Zhang
May 3, 2013·PloS One·Ying YuYanjin Zhang
Nov 19, 2014·BMC Microbiology·Chunhe GuoZuyong He
May 11, 2011·Antiviral Research·Tanja OpriessnigYan-Jin Zhang
Mar 9, 2010·Virus Research·Jianqiang ZhangUdeni B R Balasuriya
Apr 14, 2016·Veterinary Microbiology·Yueqiang XiaoYan-Jin Zhang
Aug 21, 2013·Veterinary Microbiology·Rong WangYan-Jin Zhang
Jun 24, 2015·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Xiaohong LiuYaosheng Chen
Sep 26, 2013·Vaccine·Rong WangYan-Jin Zhang
Jun 19, 2012·Virology·Yuchen NanYan-Jin Zhang
Jun 6, 2015·PloS One·Gang WangXuehui Cai
Nov 3, 2016·Scientific Reports·Zexu MaYan-Jin Zhang
Jul 13, 2017·The Journal of General Virology·Zexu MaYan-Jin Zhang
Nov 25, 2016·Journal of Virology·Liping YangYan-Jin Zhang