Morphologic changes in boar sperm nuclei with reduced disulfide bonds in electrostimulated porcine oocytes

Reproduction : the Official Journal of the Society for the Study of Fertility
Michiko NakaiK Kikuchi

Abstract

In pigs, failure of sperm nuclear decondensation has been reported after injection into oocytes. We examined the effects of pretreating sperm heads with Triton X-100 (TX-100) and dithiothreitol (DTT) and of electrical stimulation of oocytes after sperm head injection on time-dependent morphologic changes in sperm nuclei and in vitro development to the blastocyst stage. In experiment 1, spermatozoa were pretreated with 1% TX-100 and 5 mM DTT (T + D) or not treated, and then injected into in vitro matured oocytes. Electrical stimulation (1.5 kV/cm, 20 mus DC pulse) was applied to the oocytes 1 h after injection (stimulated group) or was not applied (unstimulated group). Some of the oocytes in each group were evaluated at hourly intervals until 10 h after injection for morphologic changes in the sperm nuclei. Unstimulated oocytes injected with untreated spermatozoa showed a delayed peak in the rate of nuclear decondensation (39.4-44.1%, 3-6 h after injection) compared with oocytes injected with T + D-treated spermatozoa (57.0% and 52.6%, 1 and 2 h, respectively). The rate of male pronucleus formation peaked 6 h after stimulation (by 40-60%) after injected oocytes had been stimulated with an electrical pulse, irrespective of whethe...Continue Reading

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Citations

May 10, 2011·The Journal of Reproduction and Development·Michiko NakaiKazuhiro Kikuchi
Mar 28, 2012·The Journal of Reproduction and Development·Tamás SomfaiTakashi Nagai
Oct 17, 2007·The Journal of Reproduction and Development·Mayuko KuromeHiroshi Nagashima
Dec 18, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Michiko NakaiKazuhiro Kikuchi
Dec 4, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Hiroyuki WatanabeYutaka Fukui
May 26, 2011·Reproduction : the Official Journal of the Society for the Study of Fertility·Michiko NakaiKazuhiro Kikuchi
Jul 18, 2014·Reproduction : the Official Journal of the Society for the Study of Fertility·Jingyu LiZhonghua Liu
Jul 25, 2008·Reproduction in Domestic Animals = Zuchthygiene·K KikuchiH Kaneko
Oct 25, 2016·Animal Science Journal = Nihon Chikusan Gakkaihō·Nguyen Thi MenAtsushi Tajima
Jan 16, 2009·Reproduction in Domestic Animals = Zuchthygiene·N MaedomariN Kashiwazaki
Apr 4, 2014·The Journal of Reproduction and Development·Michiko NakaiKazuhiro Kikuchi
Oct 12, 2016·The Journal of Reproduction and Development·Michiko NakaiKazuhiro Kikuchi
Dec 3, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·Daniel F SalamoneMaría Belén Rodríguez
Jul 10, 2018·The Journal of Reproduction and Development·Hiroyuki Watanabe

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