Morphologic changes in photodamaged organotypic human skin culture after treatment of autologous adipose-derived stromal cells

The Journal of Craniofacial Surgery
Eun-Sang DhongKeith L March

Abstract

Recent clinical trials indicate that human adipose-derived stromal cells (hASCs) have beneficial effects on antiaging and wound healing. This study examined the morphologic changes in photodamaged human organotypic skin culture after treatment of autologous hASCs. Abdominal skin flaps were obtained from 8 white females who underwent abdominoplasties with liposuction. The adipose layer was removed and used for hASC isolation. Sections of skin were removed and cultured in serum-free medium. To induce photodamage, some of the skin pieces were irradiated with maximum subcytotoxic doses of UVB (1600 J/m(2)) and UVA (250 J/m(2)). The effects of hASC on skin segments were evaluated by coculture as a feeder layer or by injecting intradermally. Portions of the skin samples were removed for analysis on days 3, 5, 7, and 9 of culture and analyzed histologically for morphology, viability, and proliferation status. Epidermal necrosis of irradiated skin was significantly reduced by the presence of hASCs. Increased parakeratosis was observed at early time points, and apoptosis in epidermis was markedly decreased by hASCs. Differences were observed in epidermal differentiation but not basal cell proliferation. Similar results were obtained by ...Continue Reading

References

Sep 24, 2005·Expert Reviews in Molecular Medicine·Christelle Adolphe, Brandon Wainwright
Sep 27, 2005·Experimental Gerontology·V J Cristofalo
Dec 21, 2005·Chemical Society Reviews·Desmond J Tobin
Aug 29, 2006·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·Alena SvobodovaJitka Vostalova

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