Morphological analysis of biofilm of peritoneal dialysis catheter in refractory peritonitis patient

CEN Case Reports
Tetsuro KusabaYoshihiro Kajita

Abstract

A 66-year-old man undergoing peritoneal dialysis (PD) was admitted to our hospital for treatment of PD-related peritonitis. Culture of the PD fluid revealed the presence of Citrobacter freundii, and therapy with ceftazidime was started intraperitoneally. The cell count in PD fluid slowly decreased over time during the first 2 weeks of treatment, but increased again on the 14th hospital day. A second culture of the PD fluid revealed the presence of Enterococcus species. A switch in antibiotic therapy to vancomycin did not improve the cell count in the PD fluid. A third culture of the PD fluid revealed the presence of Stenotrophomonas maltophilia. The PD was discontinued and the catheter removed on the 28th hospital day. Examination of the catheter revealed that the inner tip was coated with a fibrous sheet of cells, suggesting biofilm formation. Following catheter removal, the patient was administered intravenous ciprofloxacin, and the inflammatory reaction started to disappear immediately and had completely disappeared after 1 week of treatment. Microscopic analysis of the fibrous structure on the catheter revealed multiple layers of various inflammatory cells. Immunostaining revealed the presence of CD44-positive polynuclear c...Continue Reading

References

Nov 1, 1990·Antimicrobial Agents and Chemotherapy·H AnwarJ W Costerton
Jun 1, 1987·Antimicrobial Agents and Chemotherapy·R C Evans, C J Holmes
Oct 1, 1982·The Journal of Infectious Diseases·G PetersG Pulverer
Jun 5, 2002·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Emily S FinkelsteinFrank J Bia
Nov 11, 2003·Scandinavian Journal of Infectious Diseases·Naveen GuptaD R Arora

❮ Previous
Next ❯

Citations

Nov 7, 2019·Peritoneal Dialysis International : Journal of the International Society for Peritoneal Dialysis·Claudia-Denise Haivas, Isaac Teitelbaum
May 28, 2021·Clinical Microbiology Reviews·Joanna S Brooke

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.