Morphological analysis of endocytosis in efferent ductules of estrogen receptor-alpha knockout male mouse

The Anatomical Record
M NakaiR A Hess

Abstract

Lack of estrogen receptor (ER) results in fluid accumulation and dilation of the efferent ductules, suggesting that the role of estrogen and ER in the male reproductive tract is related to fluid reabsorption in the ductules. In the present study, endocytosis of the nonciliated cells of the efferent ductules was compared morphologically between wild type (WT) and estrogen receptor-alpha knockout (alpha ERKO) male mice. The epithelial cells lining the WT efferent ductules were tall columnar in shape, whereas those of the alpha ERKO were low columnar. Immunocytochemically, the nonciliated cells of both genotypes showed positive reactions of sulfated glycoprotein-2, but the reaction products were reduced in amount in the alpha ERKO. Electron microscopy revealed that the nonciliated cells of the WT had numerous organelles for endocytosis such as coated pits and vesicles, tubules, endosomes, multivesicular bodies and lysosomes in the apical cytoplasm. These organelles were less developed in the nonciliated cells of the alpha ERKO. Morphometric analysis indicated that there was a significant reduction in area of endocytotic apparatus in the nonciliated cells of the alpha ERKO compared with that of the WT. A tracer study using gold par...Continue Reading

References

Nov 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·G A Ackerman, W H Freeman
Oct 1, 1992·The Anatomical Record·K Y Ilio, R A Hess
Sep 1, 1984·The American Journal of Anatomy·L Hermo, C Morales
Dec 15, 1994·Microscopy Research and Technique·K Y Ilio, R A Hess
Dec 11, 1997·Nature·R A HessD B Lubahn
Jun 11, 1999·Experimental Physiology·L A HansenR C Jones

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Citations

Jul 20, 2006·Anatomy and Embryology·P C OzegbeJ T Soley
May 15, 2002·Fertility and Sterility·Evan R Simpson
Jun 19, 2001·Molecular and Cellular Endocrinology·R A HessJ Bahr
Nov 19, 2003·The Journal of Steroid Biochemistry and Molecular Biology·E R Simpson
Oct 12, 2010·Biology of Reproduction·Avenel JosephRex A Hess
Apr 21, 2010·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Serge Carreau, Rex A Hess
Sep 16, 2004·Molecular and Cellular Biology·Ben DaviesUlrich Gottwald
Aug 9, 2003·Reproductive Biology and Endocrinology : RB&E·Rex A Hess
Feb 10, 2006·International Journal of Andrology·M K O'Bryan, D de Kretser
Feb 14, 2018·Biology of Reproduction·Rex A Hess, Paul S Cooke
May 26, 2017·Physiological Reviews·Paul S CookeRex A Hess
Sep 10, 2010·Biology of Reproduction·Gisele Renata Oliveira GomesCatarina Segreti Porto
May 23, 2008·Biology of Reproduction·Fabiana YasuharaMaria Fatima Magalhaes Lazari
Jan 15, 2021·Differentiation; Research in Biological Diversity·Rex A HessBarry T Hinton

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