Morphological and chemical optimization of microcantilever surfaces for thyroid system biosensing and beyond

Analytica Chimica Acta
Kasey HillMichael J Sepaniak

Abstract

The development of biosensors is vital in many areas of biotechnology and biomedical research. A prominent new class of label-free biosensors are those based on ligand-induced nanomechanical responses of microcantilevers (MCs). The interaction between biologically significant ligands with bioreceptors (e.g., antibodies or nuclear receptor proteins) immobilized on one side of the MC surface causes an apparent surface stress, resulting in static bending of the MC, which can be detected by an optical beam bending technique. The three key performance metrics of sensitivity, selectivity, and reversibility are foci of the work reported herein. The nature of the MC surface and the method by which the bioreceptor is immobilized influence these performance metrics and, hence, optimization studies involving these were conducted. In our work, the gold surface on one side of the MC is first activated via self-assembled monolayer formation with amino ethane thiol (AET) then reacted with glutaraldehyde (GA) as a crosslinker before finally functionalizing with the protein receptor. We report the effect of concentration, reaction time, and pH for these reagents on the magnitude of the nanomechanical responses using an anti-immunoglobulin G (an...Continue Reading

Citations

Mar 12, 2011·The Analyst·Koutilya R BuchapudiThomas Thundat
Dec 2, 2017·Advanced Healthcare Materials·Pranjal NautiyalArvind Agarwal
Apr 30, 2010·Analytical Chemistry·Zhou LongMichael J Sepaniak

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