Abstract
Triglyceride-rich rat lipoproteins (mainly VLDL), purified by ultracentrifugation and dialysis, were incubated for 120 min in the presence of epididymal fat-pad pieces from fed rats in media containing heparin. During this process, the lipoproteins were depleted of triglycerides and proportionally enriched with proteins, causing their increased density. After incubation, electron microscopic study of the triglyceride-rich lipoproteins revealed partially degraded structures, and some particles had atypical discoid flattened form and marked lamellar structure. These findings are in agreement with those of Blanchette-Mackie & Scow (1976) in triglyceride hydrolysed chylomicrons and confirm their observation of monolayers formed by accumulated lipolytic products that move by lateral diffusion, giving rise to a spiral fold between the core triglycerides and the aqueous space.
References
Sep 1, 1975·The Journal of Clinical Investigation·O D MjosR J Havel
Feb 27, 1978·Biochimica Et Biophysica Acta·L DoryD Rubinstein
Jan 1, 1978·Annual Review of Biochemistry·L C SmithA M Gotto
Aug 1, 1976·Circulation Research·R O ScowL C Smith
Feb 1, 1975·Analytical Biochemistry·C Wang, R L Smith
Oct 1, 1971·The Journal of Cell Biology·E J Blanchette-Mackie, R O Scow
Feb 21, 1972·Biochimica Et Biophysica Acta·D W BilheimerR I Levy
Dec 20, 1973·Biochimica Et Biophysica Acta·S EisenbergF T Lindgren
Sep 1, 1973·The Journal of Cell Biology·E J Blanchette-Mackie, R O Scow
Sep 1, 1971·The Journal of Clinical Investigation·E N BergmanT Bohmer
Aug 1, 1966·Metabolism: Clinical and Experimental·B PerssonB Hood
Aug 1, 1980·Archives internationales de physiologie et de biochimie·S Carmaniu, E Herrera
May 25, 1959·The Journal of Biophysical and Biochemical Cytology·W STOECKENIUS
Dec 8, 1962·Nature·P B GARLAND, P J RANDLE