Morphological and Functional Alterations of Alveolar Macrophages in a Murine Model of Chronic Inflammatory Lung Disease

Lung
Julia Désirée BoehmeDunja Bruder

Abstract

Chronic lung inflammation commonly induces a multitude of structural and functional adaptations within the lung tissue and airspaces. Yet the impact of a persistent inflammatory environment on alveolar macrophages is still incompletely understood. Here, we examined morphology and function of alveolar macrophages in a transgenic mouse model of chronic lung disease. Imaging flow cytometry, flow cytometry, and microscopic evaluation of alveolar macrophages isolated from healthy and inflamed lungs were performed. Gene expression of polarization markers was compared by quantitative real-time RT-PCR. The pro-inflammatory immune response of alveolar macrophages toward bacterial ligands was assessed in in vivo clodronate-liposome depletion studies. Chronic lung inflammation is associated with a substantially altered, activated alveolar macrophage morphology, and blunted TNF-α response by these cells following stimulation with ligands derived from the respiratory pathogen Streptococcus pneumoniae. These results demonstrate pleiotropic effects of pulmonary inflammation on alveolar macrophage phenotype and function and suggest a functional impairment of these cells during infection with airborne pathogens.

References

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Dec 20, 2008·American Journal of Respiratory and Critical Care Medicine·Marcus GerekeDunja Bruder
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Oct 9, 2013·Proceedings of the National Academy of Sciences of the United States of America·Frances Y McWhorterWendy F Liu

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Citations

Nov 16, 2019·Nanotoxicology·Massimiliano G BianchiEnrico Bergamaschi
Jul 12, 2017·Scientific Reports·Julia D BoehmeDunja Bruder
Apr 3, 2021·The Journal of Trauma and Acute Care Surgery·Mark BarryShibani Pati

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Methods Mentioned

BETA
transgenic
Flow
Bronchoalveolar lavage
flow cytometry
Fluorescence
light microscopy
FACS
ELISA

Software Mentioned

FlowSight
Graph Pad Prism
IDEAS
Inspire
ZEN

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