PMID: 42207Oct 1, 1979

Morphological and functional characteristics of cells infiltrating and destroying tumor multicellular spheroids in vivo

Virchows Archiv. B, Cell Pathology Including Molecular Pathology
E M LordR A Cooper


EMT6 mammary sarcoma cells were grown in vitro as multicellular spheroids to model for the heterogeneity of microenvironments and structural changes which develop in many tumors, including micrometastases. Spheroids of 700-900 micron diameter were implanted into and recovered at different times from the peritoneal cavities of sensitized or nonsensitized allogeneic and syngeneic mice. The colony forming efficiency of spheroid tumor cells recovered at 24 and 48 h from sensitized allogeneic mice was markedly decreased as compared with those from nonsensitized allogeneic or syngeneic animals. These recovered spheroids were extensively infiltrated by both lymphocytes and macrophages, which ultrastructurally had very close membrane associations with tumor cells. Host cells recovered from spheroids exhibited cytotoxic activity in an in vitro 51Cr release assay. Thus, multicellular spheroids in vivo provide a unique experimental model to study the functional capacity of host cells within a spheroical tumor. Although lacking the stroma and the vasculature of in vivo solid tumors, this model does have many similarities to in vivo tumors and is thus suitable for studying the tumor cell-host cell interactions within the tumor microenvironm...Continue Reading


Jan 20, 1976·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·C J Sanderson
Jun 1, 1977·Journal of the National Cancer Institute·R M SutherlandR L Howell
Sep 15, 1976·International Journal of Cancer. Journal International Du Cancer·S W RussellC G Cochrane
Nov 1, 1977·International Journal of Radiation Oncology, Biology, Physics·D P Penney, P Rubin
Jan 1, 1974·Advances in Immunology·J C Cerottini, K T Brunner
May 1, 1974·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·H S Kaplan
Jan 1, 1974·Advances in Cancer Research·R B Herberman
Jul 1, 1972·Clinical Radiology·J F Fowler
Mar 1, 1971·The Japanese Journal of Surgery·S FujimotoK Okahara


Oct 1, 1989·Cellular Immunology·K M WilsonE M Lord
Aug 1, 1981·International Journal of Radiation Oncology, Biology, Physics·J B Beecham, S S Knauf
Jan 1, 1981·Journal of Cellular Physiology·J LandryR M Sutherland
Jan 1, 1987·Journal of Cancer Research and Clinical Oncology·W Mueller-Klieser
Jul 28, 2020·Experimental Biology and Medicine·Xiaopeng CaiZhi Chen

Related Concepts

Lymphoid Cells
Mammary Neoplasms, Experimental
Mice, Inbred Strains
Greater Sac of Peritoneum
Sarcoma, Jensen

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