Morphological and Genetic Diversity of Rhizobia Nodulating Cowpea (Vigna unguiculata L.) from Agricultural Soils of Lower Eastern Kenya

International Journal of Microbiology
Damaris K OndiekiFrancis B Mwaura

Abstract

Limited nitrogen (N) content in the soil is a major challenge to sustainable and high crop production in many developing countries. The nitrogen fixing symbiosis of legumes with rhizobia plays an important role in supplying sufficient N for legumes and subsequent nonleguminous crops. To identify rhizobia strains which are suitable for bioinoculant production, characterization of rhizobia is a prerequisite. The objective of this study was to assess the morphological and genetic diversity of rhizobia that nodulates cowpea in agricultural soils of lower eastern Kenya. Twenty-eight rhizobia isolates were recovered from soil samples collected from farmers' fields in Machakos, Makueni, and Kitui counties in lower eastern Kenya and characterized based on morphological characteristics. Thirteen representative isolates were selected and characterized using BOX repetitive element PCR fingerprinting. Based on the dendrogram generated from morphological characteristics, the test isolates were distributed into two major clusters at a similarity of 75%. Phylogenetic tree, based on BOX repetitive element PCR, grouped the isolates into two clusters at 90% similarity level. The clustering of the isolates did not show a relationship to the origi...Continue Reading

References

May 15, 1999·Journal of Clinical Microbiology·D M Olive, P Bean
Apr 12, 2000·International Journal of Systematic and Evolutionary Microbiology·J L RademakerF J de Bruijn
Oct 26, 2002·Microbiological Research·Sanja SikoraMihaela Bradić
Jan 7, 2005·Journal of Clinical Microbiology·Mimi HealyJames R Lupski
May 5, 2007·Archives of Microbiology·Chang Fu TianWen Xin Chen
Mar 18, 2009·Applied Microbiology and Biotechnology·Daisy Rickli BindeMariangela Hungria
Jul 17, 2012·Applied and Environmental Microbiology·Amanda Azarias GuimarãesFatima Maria de Souza Moreira

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Citations

Oct 6, 2020·Frontiers in Microbiology·Olubukola Oluranti BabalolaAyansina S Ayangbenro
Feb 13, 2021·Scientific Reports·Bayou Bunkura AllitoDemelash Kefale Hunegnaw
Jun 15, 2021·Systematic and Applied Microbiology·Margarida G SimbineFelix D Dakora

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Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

DNR
SPSS

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