Morphological and histochemical studies on a PAS-positive granular leukocyte in blood and connective tissues of Catostomus commersonii Lacépède (teleostei:pisces)

The American Journal of Anatomy
D L Barber, J E Westermann

Abstract

This study was undertaken to identify an ubiquitous granular leukocyte found in Catostomus commersonni Lacépède. The cell contains large, numerous, strongly PAS-positive cytoplasmic granules, an eccentric nucleus and prominent, persistent juxtanuclear space. It develops in the hemopoietic tissue of the kidney, and mature cells are found not only in kidney and peripheral blood but also in areas of connective tissue where mast cells are usually located. Electron microscopy confirms the presence of a large Golgi apparatus, unlamellated cytoplasmic granules and extensive rough-surfaced endoplasmic reticulum. Histochemical studies show that the cytoplasmic granules are alcianophobic, non-metachromatic and unstained by acridine orange. Histamine is detectable spectrophotometrically in kidney tissue, but the PAS-positive granular leukocyte does not consistently degranulate after treatment with histamine liberator 48/80. The authors suggest that while the PAS-positive granular leukocyte is not identical with classical basophils/mast cells, which are absent in C. commersonnii, it may represent an evolutionary precursor of these cells.

Citations

Oct 1, 1965·Histochemie. Histochemistry. Histochimie·J E Scott, J Dorling
Jul 1, 1970·The Anatomical Record·A M SmithM Potter
May 1, 1954·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·H KRAMER, G M WINDRUM
Jul 1, 1957·Stain Technology·K HARADA

Related Concepts

Ovoperoxidase
Reticulopodia
Golgi Apparatus
Mast Cell
Liquaemin
Kidney
Cell Nucleus
Leukocytes
Electron Microscopy
Connective Tissue Cells

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