Morphological and microarray analysis of T-2 toxin-induced rat fetal brain lesion

Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association
Shinya SehataKunio Doi

Abstract

To examine morphological and gene expression changes induced by T-2 toxin in the fetal brain in detail, pregnant rats on day 13 of gestation were treated orally with a single dose of T-2 toxin (2 mg/kg) and sacrificed at 1, 3, 6, 9, 12 and 24 h after treatment (HAT). Histopathologically, the number of apoptotic neuroepithelial cells in the telencephalon increased from 1 HAT and peaked at 12 HAT. Based on the histopathological examinations, microarray analysis was performed at 6, 12 and 24 HAT. Microarray analysis showed that the expression of oxidative stress-related genes (heat shock protein 70 (HSP70) and heme oxygenase (HO)) was strongly induced by T-2 toxin at 12 HAT, the peak time point of apoptosis induction. The expression of mitogen-activated protein kinase (MAPK)-related genes (MEKK1 and c-jun) and other apoptosis-related genes (caspase-2 and insulin-like growth factor-binding protein-3 (IGF-BP3)) was also induced by the T-2 toxin treatment. The changes observed by microarray analysis were confirmed for four up-regulated genes (HSP70, HO, IGF-BP3 and VEGF-A) using real-time RT-PCR. Our results suggest that the T-2 toxin-induced apoptosis in the fetal brain is due to oxidative stress, and that the MAPK pathway may be in...Continue Reading

References

Sep 15, 1990·Toxicology and Applied Pharmacology·W L Thompson, R W Wannemacher
Jan 1, 1986·Toxicon : Official Journal of the International Society on Toxinology·L J MartinA Anthony
Sep 15, 1989·Biochemical Pharmacology·J L Middlebrook, D L Leatherman
Aug 1, 1987·Journal of Applied Toxicology : JAT·C G Rousseaux, H B Schiefer
Aug 1, 1982·Journal of Applied Toxicology : JAT·M A Hayes, H B Schiefer
Aug 15, 1997·The Biochemical Journal·G M Cohen
Oct 15, 1998·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·J WangJ R Wilson
Feb 15, 2000·The Journal of Biological Chemistry·M R CirioloG Rotilio
Apr 15, 2000·Bioscience, Biotechnology, and Biochemistry·A M MurshedulN Sakato
Aug 30, 2000·Proceedings of the National Academy of Sciences of the United States of America·K L JinD A Greenberg
Sep 29, 2000·Biochemical Pharmacology·J HamB Shah
Mar 21, 2001·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·N IshigamiK Doi
Mar 29, 2001·Food Additives and Contaminants·P GuerreP Galtier
May 9, 2001·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S J BuleraF A De La Iglesia
Jul 27, 2001·Brain Research Bulletin·J HidalgoM Vasák
Aug 18, 2001·Toxicology and Applied Pharmacology·J F WaringR G Ulrich
Aug 21, 2001·Biochemical and Biophysical Research Communications·C GarridoG Kroemer
Oct 23, 2001·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·S M AlbarenqueK Doi
Jan 5, 2002·The Journal of Biological Chemistry·Omolara O OgunsholaLaura R Ment
Feb 5, 2003·Experimental Cell Research·Simone BoldtWalter Kolch
Mar 26, 2003·Cell Death and Differentiation·C M Troy, M L Shelanski
Apr 20, 2004·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Shinya SehataKunio Doi

❮ Previous
Next ❯

Citations

Sep 29, 2011·International Journal of Molecular Sciences·Kunio Doi, Koji Uetsuka
Mar 31, 2009·International Journal of Molecular Sciences·Kunio DoiShinya Sehata
Nov 10, 2013·The Journal of Veterinary Medical Science·Jing SunSatoshi Furukawa
Mar 31, 2009·International Journal of Molecular Sciences·Yumiko IwahashiHitoshi Iwahashi
Dec 29, 2015·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Zhihang YuanJing Wu
Mar 30, 2007·Toxicology Letters·Peixin HuangKanehisa Morimoto
Jun 11, 2005·Journal of Neuroscience Research·Peter J Gebicke-Haerter
Jun 16, 2009·Journal of Biochemical and Molecular Toxicology·Manjari ChaudhariP V Lakshmana Rao
Jul 19, 2005·Toxicology Letters·Zaharenia VlataElias Krambovitis
Jul 5, 2015·Toxicological Sciences : an Official Journal of the Society of Toxicology·Dan WanZonghui Yuan
Nov 27, 2014·Toxicology and Industrial Health·Bence SomoskőiSándor Cseh
Apr 6, 2019·Environmental Toxicology·Kristina Pogrmic-MajkicNebojsa Andric
Aug 30, 2008·PloS One·Jean-Marc MelgariPaolo Maria Rossini
Nov 2, 2011·European Journal of Neurology : the Official Journal of the European Federation of Neurological Societies·M KönönenR Vanninen
Mar 28, 2020·PloS One·Shivasharanappa NayakwadiKanthesh M Basalingappa
Jun 20, 2018·Toxins·Alessia BerteroFrancesca Caloni
Jan 15, 2016·The Journal of Toxicological Sciences·Takahito ToyotomeKatsuhiko Kamei
Dec 31, 2019·BioMed Research International·Yuqi ChenHuadong Yin
Aug 18, 2020·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Jiajia ZhangKamil Kuca
May 2, 2021·Environmental Science and Pollution Research International·Sara ShahbaAmir Mohammad Malvandi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.

Advanced Imaging of Cellular Signaling

Cell signaling is a vital mechanism for communication within cells and outside with the environment. Several different signaling pathways have been found and advanced imaging techniques are being developed to visualize the molecules involved in these signaling pathways. Find the latest research in advanced imaging of cellular signaling here.

Blood Brain Barrier Chips

The blood brain barrier (BBB) is comprised of endothelial cells that regulate the influx and outflux of plasma concentrations. Lab-on-a-chip devices allow scientists to model diseases and mechanisms such as the passage of therapeutic antibodies across the BBB. Discover the latest research on BBB chips here.

Blood Brain Barrier

The blood brain barrier is a border that separates blood from cerebrospinal fluid. Discover the latest search on this highly selective semipermeable membrane here.