Morphological and molecular identification of Sarcocystis spp. from the sika deer (Cervus nippon), including two new species Sarcocystis frondea and Sarcocystis nipponi

Parasitology Research
Eglė Rudaitytė-LukošienėElena Servienė

Abstract

Diaphragm muscles of 25 sika deer (Cervus nippon) farmed in Lithuania were examined for sarcocysts of Sarcocystis species. Two new Sarcocystis species, Sarcocystis frondea and Sarcocystis nipponi, were observed using light microscopy (LM) and transmission electron microscopy (TEM) and characterized by 18S ribosomal DNA (rDNA) and subunit I of cytochrome c oxidase (cox1) sequence analyses. By LM, sarcocysts of S. frondea and S. nipponi were ribbon-shaped and had finger-like sarcocyst wall protrusions, respectively. Under TEM, protrusions of S. frondea were about 9 × 1-1.5 μm, filled with clearly visible electron-dense substance and microtubules, type 39-like. Whereas, protrusions (about 9 × 0.2 μm) of S. nipponi arose from dome-shaped bases were filled with microtubules extending to the ground substance layer, type 9o-like. Moreover, three known Sarcocystis spp., Sarcocystis entzerothi, Sarcocystis ovalis, and Sarcocystis truncata previously described in other cervids as intermediate hosts, were characterized in sika deer. The cox1 was more suitable than 18S rDNA delimitating closely related Sarcocystis species from cervids. The phylogenetic results suggest that scavenger birds could be definitive hosts of S. frondea. According ...Continue Reading

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Citations

May 24, 2019·Internal Medicine·Takayuki OtaTakahiro Ohnishi
Jan 14, 2020·Parasitology Research·E Rudaitytė-LukošienėD Butkauskas
May 24, 2019·Animals : an Open Access Journal From MDPI·Dina M MetwallyManal F El-Khadragy
Mar 22, 2019·Parasitology Research·Petras PrakasDalius Butkauskas
Nov 18, 2019·Parasitology Research·Bjørn GjerdeMónica Luzón
Dec 28, 2019·International Journal for Parasitology. Parasites and Wildlife·Takao IrieKinpei Yagi
Jul 13, 2021·Parasitology Research·Guillermo E Delgado-de Las CuevasRafael Calero-Bernal
Jul 14, 2021·Parasitology International·Eglė Rudaitytė-LukošienėDalius Butkauskas

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Datasets Mentioned

BETA
AB698065

Methods Mentioned

BETA
light microscopy
PCR

Software Mentioned

MEGA7

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