Morphological and proteomic analysis of young spikes reveals new insights into the formation of multiple-pistil wheat.

Plant Science : an International Journal of Experimental Plant Biology
Zheng LiGaisheng Zhang

Abstract

A new multiple-pistil wheat mutant germplasm with more than one pistil in a floret was obtained from natural mutagenesis. This mutant can develop 2-3 grains in a glume after pollination and has a significant grain number advantage compared with normal wheat. However, the basis of the formation of multiple-pistil wheat has thus far not been well established. In this study, we first performed a continuous phenotypic observation of the floral meristem (FM) in multiple-pistil wheat. The results indicated that the secondary pistils are derived from extra stem cells that fail to terminate normally between the carpel primordium and the lodicule primordium. To further probe the potential molecular basis for the formation of secondary pistils, comparative proteomic analyses were conducted. A total of 334 differentially abundant proteins (DAPs) were identified using isobaric tags for relative and absolute quantification (iTRAQ), among which 131 proteins were highly abundant and 203 proteins were less abundant in the young spikes of multiple-pistil wheat. The DAPs, located primarily in the cell, were involved in the translation and the metabolisms of carbohydrate, nucleotide, and amino acid. Differential expression analysis showed that Ta...Continue Reading

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