Morphological and ultrastructural evaluation of cultured frozen-thawed human fetal ovarian tissue

Fertility and Sterility
J C SadeuJ Smitz

Abstract

To investigate a defined culture condition for the culture of frozen-thawed human ovarian tissue. Prospective laboratory study. Reproductive biology laboratories in university hospitals. Fetal ovarian tissue from elective termination of pregnancy. Culture of frozen-thawed fetal ovarian tissue for up to 63 days. Morphology, morphometry, and survival of follicles in relation to culture times. The proportion of primordial, early primary, and primary follicles in frozen-thawed (day 0) ovarian tissue was 77.5%, 21.7%, and 0.8%, respectively. Pronounced degeneration was found in all cell types, and < or =36% of the follicles had signs of atresia at days 7-14, but this figure improved with culture time to <20% of the total follicular population. After 7-14 and 21-35 days of culture, the relative proportion of the follicles in the different classes remained nearly stable. Morphometric examination of healthy follicles showed a significant increase in both follicle and oocyte diameter compared with control. A few follicles had developed to the early secondary stage. Ultrastructural analysis demonstrated well-preserved morphological integrity of healthy primordial and early primary follicles. Immunohistochemical localization of proliferat...Continue Reading

References

Feb 1, 1980·The Journal of Experimental Zoology·R BachvarovaA Tejblum
Jul 1, 1995·Journal of Assisted Reproduction and Genetics·J ZhangM DiMattina
Jul 1, 1996·Human Reproduction·H NewtonR Gosden
Jan 1, 1996·Biology of Reproduction·J J Eppig, M J O'Brien
Nov 1, 1996·Biology of Reproduction·S A WandjiJ E Fortune
Jan 1, 1997·Journal of Reproduction and Fertility·R Braw-Tal, S Yossefi
Apr 29, 1998·The Journal of Clinical Endocrinology and Metabolism·M TanikawaN Terakawa
Jun 24, 1999·Molecular Endocrinology·J A ElvinM M Matzuk
Mar 25, 2000·Theriogenology·J E FortuneV Srsen
Apr 25, 2000·Molecular and Cellular Endocrinology·A H SathananthanA Trounson
Jan 13, 2001·Molecular and Cellular Endocrinology·O Hovatta
Mar 22, 2001·Biology of Reproduction·J A FlawsP A Furth
Apr 28, 2001·Molecular and Cellular Endocrinology·E NilssonM K Skinner
Jan 15, 2002·The Journal of Clinical Endocrinology and Metabolism·Julius Gisli HreinssonOuti Hovatta
Feb 28, 2002·Endocrinology·Alexandra L L DurlingerAxel P N Themmen
Jul 26, 2002·Fertility and Sterility·Ronit AbirBenjamin Fisch
Oct 22, 1963·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·T G BAKER
Mar 24, 2004·Fertility and Sterility·Tal Biron-ShentalRonit Abir
Mar 26, 2004·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Outi Hovatta
Sep 9, 2004·Reproductive Biomedicine Online·Jennifer E ScottOuti Hovatta
Mar 1, 1946·The Anatomical Record·E WITSCHI

❮ Previous
Next ❯

Citations

Feb 3, 2011·Journal of Assisted Reproduction and Genetics·Jian-Min ZhangZi-Jiang Chen
Mar 25, 2011·Journal of Assisted Reproduction and Genetics·Jian-Min ZhangZi-Jiang Chen
Aug 18, 2012·Journal of Assisted Reproduction and Genetics·Jian-Min ZhangHeng-Cai Wang
Mar 19, 2011·Zygote : the Biology of Gametes and Early Embryos·Janduí Escarião da NóbregaJoão Francisco Coelho de Oliveira
Jul 9, 2009·Molecular Human Reproduction·G M HartshorneF Ghafari
Jan 15, 2011·Reproductive Biology and Endocrinology : RB&E·Stefania A NottolaGuido Macchiarelli
Apr 25, 2007·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Artur J Jakimiuk, Wojciech Grzybowski
Aug 24, 2010·Research in Veterinary Science·M H T MatosJ R Figueiredo
Jul 12, 2008·Reproductive Biomedicine Online·Alessandra CamboniAnne Van Langendonckt
Aug 18, 2009·Fertility and Sterility·Anne PerdrixNathalie Rives
Jan 8, 2008·Gynécologie, obstétrique & fertilité·C MazoyerJ Lornage
Sep 1, 2007·Fertility and Sterility·Stefania A NottolaJacques Donnez
Jun 26, 2007·Gynécologie, obstétrique & fertilité·B CourbièreM Gamerre
May 13, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·J C SadeuJ Smitz
Jun 29, 2012·The Journal of Reproduction and Development·Yuji Hirao

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.