Morphological, Biochemical, and Functional Study of Viral Replication Compartments Isolated from Adenovirus-Infected Cells

Journal of Virology
Paloma HidalgoRamon A Gonzalez

Abstract

Adenovirus (Ad) replication compartments (RC) are nuclear microenvironments where the viral genome is replicated and a coordinated program of late gene expression is established. These virus-induced nuclear sites seem to behave as central hubs for the regulation of virus-host cell interactions, since proteins that promote efficient viral replication as well as factors that participate in the antiviral response are coopted and concentrated there. To gain further insight into the activities of viral RC, here we report, for the first time, the morphology, composition, and activities of RC isolated from Ad-infected cells. Morphological analyses of isolated RC particles by superresolution microscopy showed that they were indistinguishable from RC within infected cells and that they displayed a dynamic compartmentalization. Furthermore, the RC-containing fractions (RCf) proved to be functional, as they directed de novo synthesis of viral DNA and RNA as well as RNA splicing, activities that are associated with RC in vivo. A detailed analysis of the production of viral late mRNA from RCf at different times postinfection revealed that viral mRNA splicing occurs in RC and that the synthesis, posttranscriptional processing, and release fr...Continue Reading

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Citations

Oct 4, 2017·Molecular & Cellular Proteomics : MCP·Emigdio D ReyesMatthew D Weitzman
Feb 13, 2019·Viruses·Martin A Prusinkiewicz, Joe S Mymryk
Nov 26, 2019·FEBS Letters·Matthew CharmanMatthew D Weitzman
Nov 24, 2019·FEBS Letters·Noémie Pied, Harald Wodrich
Nov 12, 2019·FEBS Letters·Paloma Hidalgo, Ramón A Gonzalez
Nov 27, 2019·FEBS Letters·Paloma HidalgoRamón A Gonzalez
Feb 6, 2020·Viruses·Matthew Charman, Matthew D Weitzman
Mar 8, 2019·Journal of Virology·Keren Nebenzahl-SharonTamar Kleinberger
Apr 6, 2017·Scientific Reports·Mirja RamkeJames Chodosh

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