Abstract
In order to compare the effects of cocaine at morphological, basal cytotoxicity, biochemical and molecular levels, cultured mouse neuroblastoma cells (Neuro-2a) were exposed to a range of concentrations of cocaine hydrochloride. Neuroblastoma cell proliferation, evaluated by quantification of total protein content, was very sensitive to cocaine, being increasingly inhibited from 12 to 72 hr of exposure (EC(50) = 3.1 mm at 24 hr). Cytoplasmic membrane permeability to lactate dehydrogenase was not particularly increased and lysosomal function was stimulated from 0.05 to 1.5 mm, and inhibited from 2.5 mm. A shift to anaerobiosis was detected as intracellular lactate dehydrogenase (LDH) activity was increased and mitochondrial succinate dehydrogenase (SDH) activity decreased. Hexosaminidase (HEX), a lysosomal enzyme involved in sphingolipid degradation, was stimulated only at 1 mm and neural acetylcholinesterase (AChE) activity was stimulated from 2.5 mm. Morphological examination of exposed cultures revealed that most cells became bipolar and multipolar neurons by extension of neurites, but also suffered cytoplasmic vacuolization, hydropic degeneration and nuclear pyknosis. Although cells developing apoptosis were observed, no DNA...Continue Reading
References
May 7, 1976·Analytical Biochemistry·M M Bradford
Jun 1, 1975·Life Sciences·S C Haffke, N W Seeds
Nov 9, 1992·Biochimica Et Biophysica Acta·B M Altura, R K Gupta
Nov 25, 1983·Journal of Immunological Methods·C Korzeniewski, D M Callewaert
Mar 16, 1984·Journal of Immunological Methods·U Landegren
Dec 16, 1983·Journal of Immunological Methods·T Mosmann
Jan 1, 1995·Pharmacology & Therapeutics·M SchwarzH Soreq
Oct 1, 1994·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M CascalesL Boscá
Sep 6, 1994·Toxicology·G RepettoM Repetto
Mar 1, 1994·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·C Göldlin, U A Boelsterli
Mar 1, 1994·The Journal of Clinical Investigation·D ZachorI Ocrant
Apr 2, 1993·Neuroscience Letters·W A Cass, N R Zahniser
Jan 1, 1997·Journal of Toxicology. Clinical Toxicology·M R Repetto, M Repetto
Jul 1, 1961·Biochemical Pharmacology·G L ELLMANR M FEATHER-STONE
Apr 1, 1996·Toxicology in Vitro : an International Journal Published in Association with BIBRA·C Yuan, D Acosta
Jan 1, 1988·Toxicology in Vitro : an International Journal Published in Association with BIBRA·E BorenfreundN Martin-Alguacil
Mar 1, 1992·Toxicology in Vitro : an International Journal Published in Association with BIBRA·X PonsodaM J Gómez-Lechón
Jul 1, 1993·Toxicology in Vitro : an International Journal Published in Association with BIBRA·E WalumL Odland
Jul 1, 1993·Toxicology in Vitro : an International Journal Published in Association with BIBRA·G RepettoM Repetto
Citations
Jan 27, 2015·PloS One·Ramesh B BadisaCarl B Goodman
Feb 11, 2018·Scientific Reports·Ramesh B BadisaCarl B Goodman
Sep 14, 2018·Cell Death Discovery·Ramesh B BadisaCarl B Goodman
Jun 30, 2019·Scientific Reports·Ramesh B BadisaCarl B Goodman