Morphological changes in amphibian and fish cell lines infected with Andrias davidianus ranavirus

Journal of Comparative Pathology
X C GaoQ Y Zhang

Abstract

Andrias davidianus ranavirus (ADRV) is an emerging viral pathogen that causes severe disease in Chinese giant salamanders, the largest extant amphibian in the world. A fish cell line, Epithelioma papulosum cyprinid (EPC), and a new amphibian cell line, Chinese giant salamander spleen cell (GSSC), were infected with ADRV and observed by light and electron microscopy. The morphological changes in these two cell lines infected with ADRV were compared. Cytopathic effect (CPE) began with rounding of the cells, progressing to cell detachment in the cell monolayer, followed by cell lysis. Significant CPE was visualized as early as 24 h post infection (hpi) in EPC cells and at 36 hpi in GSSC cells. Microscopical examination showed clear and significant CPE in EPC cells, while less extensive and irregular CPE with some adherent cells remaining was observed in GSSC cells. Following ADRV infection, CPE became more extensive. Transmission electron micrographs showed many virus particles around cytoplasmic vacuoles, formed as crystalline arrays or scattered in the cytoplasm of infected cells. Infected cells showed alteration in nuclear morphology, with condensed and marginalized nuclear chromatin on the inner aspect of the nuclear membrane ...Continue Reading

References

Feb 7, 2001·Archives of Virology·A D HyattB E Coupar
Dec 17, 2011·Emerging Infectious Diseases·Wuzi DongWenxian Zeng
Jan 17, 2015·Science China. Life Sciences·QiYa Zhang, Jian-Fang Gui

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Citations

Feb 19, 2016·Journal of Immunoassay & Immunochemistry·Xiao-Chan GaoQi-Ya Zhang
Dec 6, 2018·Frontiers in Microbiology·Jia LiuQi-Ya Zhang

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