PMID: 1293508Dec 16, 1992

Morphological changes in Krebs II ascites tumour cells induced by insulin are associated with differences in protein composition and altered amounts of free, cytoskeletal-bound and membrane-bound polysomes

Molecular and Cellular Biochemistry
E K KirkeeideA Vedeler

Abstract

A three-step sequential detergent/salt extraction procedure was used in order to isolate three distinct subcellular fractions containing free (FP), cytoskeletal-bound (CBP) and membrane-bound polysomes (MBP), respectively, from Krebs II ascites cells (Vedeler et al., Mol Cell Biochem 100: 183-193, 1991). The purpose was to study changes in the distribution of polysomes in these three fractions during long-term incubation with insulin under either stationary conditions or in roller suspension culture. Insulin caused a redistribution of polysomes between FP, CBP and MBP fractions. The hormone appeared to promote an entry of ribosomes into polysomes both in CBP and MBP populations. When cells were grown in stationary culture in the presence of insulin and thus promoted to attach to the substratum and undergo morphological changes, a diversion of ribosomes from CBP into MBP was observed. The level of protein synthesis was apparently very high in this latter fraction since more than 70% of ribosomes were in polysomes. Morphological changes observed following insulin treatment were accompanied by a shift of certain proteins among subcellular fractions (for example actin and p35). The fibronectin content was about 20% higher in attach...Continue Reading

References

Feb 1, 1992·The International Journal of Biochemistry·B AlmåsJ Hesketh
Nov 13, 1991·Molecular and Cellular Biochemistry·A VedelerJ Hesketh
Nov 1, 1991·Biochemical Society Transactions·A VedelerJ Hesketh
Jul 1, 1991·The Biochemical Journal·J Hesketh, I F Pryme
Mar 1, 1991·The Biochemical Journal·J HeskethP F Whitelaw
Dec 2, 1990·Cell Differentiation and Development : the Official Journal of the International Society of Developmental Biologists·F J Fogerty, D F Mosher
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May 17, 1990·Nature·M J Crumpton, J R Dedman

Citations

Jun 1, 1993·The International Journal of Biochemistry·E K KirkeeideA Vedeler
Jul 23, 2014·Physical Biology·Seyed Jamaleddin Mousavi, Mohamed Hamdy Doweidar

Related Concepts

Ascites
Cell Adhesion
Microtrabecular Lattice
Fibronectins
Novolin
Mice, Inbred BALB C
Polyribosomes
Ribonucleoproteins
Ribosomes

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