Morphological changes induced by phospholipase C and by sphingomyelinase on large unilamellar vesicles: a cryo-transmission electron microscopy study of liposome fusion

Biophysical Journal
G BasáñezK Edwards

Abstract

Cryo-transmission electron microscopy has been applied to the study of the changes induced by phospholipase C on large unilamellar vesicles containing phosphatidylcholine, as well as to the action of sphingomyelinase on vesicles containing sphingomyelin. In both cases vesicle aggregation occurs as the earliest detectable phenomenon; later, each system behaves differently. Phospholipase C induces vesicle fusion through an intermediate consisting of aggregated and closely packed vesicles (the "honeycomb structure") that finally transforms into large spherical vesicles. The same honeycomb structure is also observed in the absence of enzyme when diacylglycerols are mixed with the other lipids in organic solution, before hydration. In this case the sample then evolves toward a cubic phase. The fact that the same honeycomb intermediate can lead to vesicle fusion (with enzyme-generated diacylglycerol) or to a cubic phase (when diacylglycerol is premixed with the lipids) is taken in support of the hypothesis according to which a highly curved lipid structure ("stalk") would act as a structural intermediate in membrane fusion. Sphingomyelinase produces complete leakage of vesicle aqueous contents and an increase in size of about one-thi...Continue Reading

Citations

Mar 10, 2001·Biopolymers·R M Epand, R F Epand
Jul 27, 2000·Journal of Cellular Physiology·R N KolesnickA Alonso
Mar 30, 2011·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Norman J HaugheyVeera Venkata Ratnam Bandaru
Oct 23, 1998·Biochimie·J ChopineauM Ollivon
Jul 2, 2003·Biophysical Chemistry·Nill BergstrandKatarina Edwards
Aug 2, 2003·Current Opinion in Cell Biology·Khashayar Farsad, Pietro De Camilli
Nov 5, 1997·Current Opinion in Structural Biology·V Luzzati
Jul 17, 1998·Current Opinion in Chemical Biology·B de Kruijff
Sep 29, 2001·Biomolecular Engineering·P Walde, S Ichikawa
May 23, 2001·The Biochemical Journal·B LarijaniD L Poccia
Jun 29, 2000·Annual Review of Biochemistry·R Jahn, T C Südhof
May 7, 2009·Annual Review of Biophysics·Banafshé Larijani, Dominic L Poccia
Jan 12, 2010·American Journal of Physiology. Heart and Circulatory Physiology·Jun-Xiang BaoPin-Lan Li
Feb 12, 2014·Progress in Lipid Research·Bruno M CastroLiana C Silva
Feb 25, 2010·FEBS Letters·Branka Stancevic, Richard Kolesnick
Jan 2, 2010·FEBS Letters·F-Xabier ContrerasFélix M Goñi
Apr 17, 2012·Progress in Lipid Research·Félix M GoñiAlicia Alonso
Oct 12, 2015·Colloids and Surfaces. B, Biointerfaces·Maitane IbargurenFélix M Goñi
May 12, 2009·Chemistry and Physics of Lipids·Patricia UrbinaFélix M Goni
Aug 8, 2007·Micron : the International Research and Review Journal for Microscopy·Sophia HatziantoniouCostas Demetzos
Feb 13, 2007·Biochimica Et Biophysica Acta·Iván López-MonteroPhilippe F Devaux
May 20, 2008·Cellular Microbiology·Michael SchrammOlaf Utermöhlen
Dec 23, 2009·Biochimica Et Biophysica Acta·Iván López-MonteroPhilippe F Devaux
Jan 3, 2012·Journal of Microencapsulation·Sagida BibiYvonne Perrie
Oct 31, 2006·Biochimica Et Biophysica Acta·Félix M Goñi, Alicia Alonso
Jul 19, 2002·Biophysical Journal·Juha M HolopainenPaavo K J Kinnunen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.