Morphological changes of the dermatophyte Trichophyton rubrum after photodynamic treatment: a scanning electron microscopy study

Medical Mycology
Threes SmijsJoke A Bouwstra

Abstract

Treatment strategies for superficial mycosis caused by the dermatophyte Trichophyton rubrum consist of the use of topical or oral antifungal preparations. We have recently discovered that T. rubrum is susceptible to photodynamic treatment (PDT), with 5,10,15-tris(4-methylpyridinium)-20-phenyl-[21H,23H]-porphine trichloride (Sylsens B) as a photosensitizer. The susceptibility appeared to depend on the fungal growth stage, with PDT efficacy higher with microconidia when compared to mycelia. The aim of this study was to investigate, with the use of scanning electron microscopy, the morphological changes caused by a lethal PDT dose to T. rubrum when grown on isolated human stratum corneum. Corresponding dark treatment and light treatment without photosensitizer were used as controls. A sub-lethal PDT dose was also included in this investigation The morphologic changes were followed at various time points after the treatment of different fungal growth stages. Normal fungal growth was characterized by a fiber-like appearance of the surface of the hyphae and microconidia with the exception of the hyphal tips in full mycelia and the microconidia shortly after attachment to the stratum corneum. Here, densely packed globular structures w...Continue Reading

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Citations

Dec 1, 2010·Photochemistry and Photobiology·Threes G M Smijs, Stan Pavel
May 23, 2015·Drug Development and Industrial Pharmacy·Avadhesh KushwahaHoward I Maibach
Sep 28, 2013·Expert Opinion on Investigational Drugs·Aditya K Gupta, Fiona C Simpson
Jan 6, 2009·Journal of the American Academy of Dermatology·Bianca Maria PiracciniAntonella Tosti
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Oct 6, 2016·Medical Mycology·Tamara AngeloTais Gratieri
Dec 12, 2017·Journal of Biophotonics·Jessica HouangAntonio Lauto
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Apr 21, 2020·Frontiers in Microbiology·Omar Y MadyAhmed A Donia
May 10, 2012·Anais Brasileiros De Dermatologia·José Cláudio Faria AmorimMarcos Pinotti
Sep 15, 2019·Journal of Microbiological Methods·Fritz Ka-Ho HoAlbert Bolhuis
Mar 7, 2021·Pathogens·Annabel GuttentagDee Carter

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