Morphological characteristics and ganglioside composition of substratum adhesion sites in a hepatoma cell line (CMH5123 cells) during different phases of growth

Experimental Cell Research
E BarlettaS Ruggieri

Abstract

This study compares the ganglioside composition of tissue culture substrate-attached material (SAM) with that of cell bodies in a line of transformed hepatocytes derived from the minimal deviation Morris hepatoma 5123 c (CMH5123 cells). We examined both confluent cultures (late-phase cultures) and cells which were allowed to attach for only 3 h (early-phase cultures). We also determined to what extent ganglioside compositions of SAM and cell bodies from early- and late-phase cultures of CMH5123 cells are affected by the block of complex ganglioside biosynthesis induced by treatment with chelating agents (EGTA + EDTA). The morphological characteristics of SAM were monitored by scanning electron microscopy during the different steps of this study. In early-phase cultures, SAM was composed of fragments of filopodia and small vesicles probably representing newly formed substratum adhesion sites. In contrast, SAM of late-phase cultures was made up of large pools of membranous material resulting from the breakage of thick retraction fibers connecting the cell body with broad, mature adhesion sites. SAM of early-phase cultures yielded ganglioside profiles with a higher content of GM1 and GD1 a than those of cell bodies, while in late-...Continue Reading

References

Jan 1, 1978·International Review of Cytology·F Grinnell
Jan 1, 1979·Journal of Supramolecular Structure·L A CulpB J Rollins
Jul 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H K KleinmanP H Fishman
Jun 1, 1977·Experimental Cell Research·J J Rosen, L A Culp
Feb 1, 1986·Experimental Cell Research·G R MatyasD J Morré
Jan 1, 1984·Toxicologic Pathology·G MugnaiS Ruggieri
Nov 1, 1981·Journal of Cellular Physiology·K M YamadaT Momoi
Nov 30, 1984·Biochemical and Biophysical Research Communications·G MugnaiS Ruggieri
Aug 1, 1981·Experimental Cell Research·C Domen, L A Culp
Feb 1, 1982·Journal of Neurochemistry·E G BremerR H McCluer
Mar 1, 1983·Archives of Biochemistry and Biophysics·L J Miketo, L A Culp

❮ Previous
Next ❯

Related Concepts

Related Feeds

Carcinoma, Hepatocellular

Hepatocellular Carcinoma is a malignant cancer in liver epithelial cells. Discover the latest research on Hepatocellular Carcinoma here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Adrenocortical Carcinoma

Hepatocellular carcinoma is the most common type of primary liver cancer and frequently occurs in individuals with chronic liver diseases like cirrhosis. Here is the latest research.