PMID: 18720852Aug 30, 2008Paper

Morphological comparison of rabies viral particles before and after rejuvenation in suckling mice and morphogenesis of the rejuvenated virus in cells

Wei sheng wu xue bao = Acta microbiologica Sinica
Xiaohu HanChangchun Tu

Abstract

To observe the morphological recovery of rabies virus strain SRV 9 after rejuvenation in suckling mice and to study its morphogenesis in BHK-21 cells. The long freeze-preserved rabies virus strain SRV 9 was rejuvenated through intracerebral inoculation of sucking mice twice, followed by propagation in BHK-21 cells. After cell culture the virus was purified through sucrose gradient density ultracentrifugation. Electromicroscopy of the purified virus showed that effective recovery of viral shape was obtained after the rejuvenation with majority of viral particles having a typical bullet-like shape and intact spikes on viral membrane. The proportion of DI particles (with short triangle and irregular shapes) in rejuvenatd virus supernatant was significantly decreased compared to un-rejuvenated virus. Viral morphogenesis in cells showed that typical virus particles could form in intracytoplasm 24 hours p.i. and the number of matured viral particles in cytoplasm increased significantly as culture was prolonged from 24 hours to 96 hours p.i.. Furthermore, the rejuvenated virus was observed budding from vacuole membrane in different patterns. (1) The proportion of DI particles can be significantly decreased by rejuvenated through intra...Continue Reading

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