Morphological Control of Microtubule-Encapsulating Giant Vesicles by Changing Hydrostatic Pressure

Biological & Pharmaceutical Bulletin
K TakiguchiMasayoshi Nishiyama

Abstract

For the development of artificial cell-like machinery, liposomes encapsulating cytoskeletons have drawn much recent attention. However, there has been no report showing isothermally reversible morphological changes of liposomes containing cytoskeletons. We succeeded in reversibly changing the shape of cell-sized giant vesicles by controlling the polymerization/depolymerization state of cytoskeletal microtubules that were encapsulated in the vesicles using pressure changes. The result indicates that it is possible to manipulate artificial cell models composed of molecules such as lipids and proteins. The findings obtained in this study will be helpful in clarifying the details of cooperation between cytoskeletal dynamics and morphogenesis of biological membranes and in improving the design and construction of further advanced artificial cell-like machinery, such as drug-delivery systems. In addition, the experimental system used in this study can be applied to research to elucidate the adaptive strategy of living organisms to external stimuli and extreme conditions such as osmotic stress and high-pressure environments like the deep sea.

References

Jun 30, 1987·Biochemistry·E T O'BrienH P Erickson
Jul 17, 1998·Journal of Molecular Biology·Y Tanaka-TakiguchiH Hotani
Feb 3, 2009·Biophysical Journal·Masayoshi NishiyamaMasahide Terazima
Aug 9, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Kingo TakiguchiKenichi Yoshikawa
Aug 23, 2011·Cell·Melissa K GardnerDavid J Odde
Jul 10, 2012·Biophysical Journal·Masayoshi Nishiyama, Yoshiyuki Sowa
Jun 7, 2014·Accounts of Chemical Research·Masami HagiyaSatoshi Murata
Aug 31, 2014·Surgical Endoscopy·Hideki HayashiHisahiro Matsubara
Jan 15, 2015·Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life·Kingo TakiguchiKenichi Yoshikawa
Jul 16, 2015·Sub-cellular Biochemistry·Masayoshi Nishiyama
Mar 30, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Masahito HayashiKingo Takiguchi
Apr 9, 2017·Methods in Molecular Biology·Masayoshi Nishiyama, Yoshiyuki Arai
Apr 24, 2017·Biophysical Chemistry·Masayoshi Nishiyama
Oct 31, 2017·Nature Chemical Biology·Zhaowei ChenZhen Gu

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